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Survival of infected red blood cell aggregates in elongational shear flow.
Lab Chip
February 2024
Institute of Physics, University of Augsburg, 86159 Augsburg, Germany.
Rosetting, the formation of red blood cell aggregates, is a life-threatening condition in malaria tropica and not yet fully understood. We study rosette stability using a set of microfluidic stenotic channels, with varied narrowing angle and erythrocytes of blood groups O and A. We find reduced ability of a rosette to pass a stenosis without disruption, the longer the tapered part of the constriction and the narrower the stenosis is.
View Article and Find Full Text PDFLectin-induced oxidative stress in human platelets.
Redox Biol
May 2020
Department of Pharmacy, Biochemistry Lab, University of Genoa, Genova, 16132, Italy. Electronic address:
Previously we have shown that wheat germ agglutinin (WGA) and, with minor potency, Phaseolus vulgaris agglutinin (PHA), but not lens culinarian agglutinin (LCA), induce platelet aggregation, through the PLCƴ2 activation by the concerted action of src/syk and PI3K/BTK pathways. In this study, we have investigated platelet oxidative stress induced by lectins. Several parameters indicative of oxidative stress, such as reactive oxygen species (ROS), superoxide anion, lipid peroxidation and the efficiency of the aerobic metabolism, have been measured.
View Article and Find Full Text PDFIdentification of (L.) Moench Root LysM Lectin with Nephrotoxic Properties.
Toxins (Basel)
January 2020
Laboratory of Cardiac Pathology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu ave. 15, 50162 Kaunas, Lithuania.
(L.) Moench (EP) is a well-studied plant used for health benefits. Even though there are a lot of data on EP secondary metabolites, its active proteins are not studied well enough.
View Article and Find Full Text PDFEffects of recombinant human lactoferrin on calcium signaling and functional responses of human neutrophils.
Arch Biochem Biophys
October 2019
Department of Biology, The University of Western Ontario, London, ON, N6A 5B7, Canada.
Lactoferrin is a non-heme iron-binding glycoprotein with multiple health-beneficial functions including antimicrobial, antioxidant, anticarcinogenic, and immunomodulatory effects. There is emerging evidence that neutrophils may serve as targets of lactoferrin in vivo, and here we show how recombinant human lactoferrin (rhLf) can contribute to this regulation. Indeed, our results demonstrate that rhLf binds efficiently to human neutrophils and induces a variety of early cellular responses such as mobilization of intracellular Ca, remodeling of actin cytoskeleton, and degranulation (release of lysozyme and myeloperoxidase).
View Article and Find Full Text PDFHuman neutrophils can mimic myeloid-derived suppressor cells (PMN-MDSC) and suppress microbead or lectin-induced T cell proliferation through artefactual mechanisms.
Sci Rep
February 2018
Division of Transplant Immunology, Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia and University of Pennsylvania, Philadelphia, PA, USA.
We report that human conventional CD15 neutrophils can be isolated in the peripheral blood mononuclear cell (PBMC) layer during Ficoll gradient separation, and that they can impair T cell proliferation in vitro without concomitant neutrophil activation and killing. This effect was observed in a total of 92 patients with organ transplants, lung cancer or anxiety/depression, and in 18 healthy donors. Although such features are typically associated in the literature with the presence of certain myeloid-derived suppressor cell (PMN-MDSC) populations, we found that commercial centrifuge tubes that contained membranes or gels for PBMC isolation led to up to 70% PBMC contamination by CD15 neutrophils, with subsequent suppressive effects in certain cellular assays.
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