AI Article Synopsis
- Low expression of NM23-H1 in head and neck squamous cell carcinoma (HNSCC) is linked to worse clinical outcomes and higher recurrence rates.
- A study involving 50 patients indicated that lower NM23-H1 levels are associated with greater chances of locoregional recurrence and poor prognosis after radiotherapy.
- Experimental results showed that knocking down NM23-H1 reduces the sensitivity of HNSCC cells to radiation, while its overexpression improves radiation response, suggesting NM23-H1 could be a therapeutic target for improving treatment outcomes.
Article Abstract
A low NM23-H1 expression in head and neck squamous cell carcinoma (HNSCC) was found to be associated with poor clinical outcome. Therefore, we investigated the role of NM23-H1 in the susceptibility of HNSCC cells to irradiation and its clinical significance. An study was also conducted to validate the results. Furthermore, we used immunohistochemistry to analyze NM23-H1 expression found in specimens of 50 HNSCC patients with cervical metastases receiving postoperative radiotherapy. Low tumor NM23-H1 expression was associated with locoregional recurrence of HNSCC (p=0.040; Hazard ratio=5.62) and poor clinical outcome (p=0.001; Hazard ratio=4.90). To confirm the effect of NM23-H1 on radiation-induced cytotoxicity, we generated several stable clones derived from a human HNSCC cell line (SAS) using knockdown and overexpression of NM23-H1. Knockdown of NM23-H1 decreased the radio-sensitivity of SAS cells, possibly associated with a decrease in the radiation-induced G2/M-phase accumulation and upregulation of cyclin B1. On the contrary, overexpression of NM23-H1 can reverse the aforementioned adverse results. Consequently, we suggest that NM23-H1 expression may be considered as a potential therapeutic treatment option for HNSCC patients.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042278 | PMC |
| http://dx.doi.org/10.3389/fonc.2021.646167 | DOI Listing |
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