Oxidase-mimicking nanozymes are more desirable than peroxidase-mimicking ones since H O can be omitted. However, only a few nanomaterials are known for oxidase-like activities. In this work, we compared the activity of Mn O , Mn O and MnO and found that Mn O had the highest oxidase activity. Interestingly, the activity of Mn O was even inhibited by H O . The oxidase-like activity of Mn O was not much affected by the presence of proteins such as bovine serum albumin (BSA), but the physisorption of antibodies to Mn O was not strong enough to withstand the displacement by BSA. We then treated Mn O with 3-aminopropyltriethoxysilane to graft an amine group, which was used to conjugate antibodies using glutaraldehyde as a crosslinker. A one-step indirect competitive ELISA (icELISA) was developed for the detection of isocarbophos, and an IC of 261.7 ng/mL was obtained, comparable with the results of the standard two-step assay using horseradish peroxidase (HRP)-labeled antibodies. This assay has the advantage of significant timesaving for rapid detection of large amounts of samples. This work has discovered a highly efficient oxidase-mimicking nanozyme useful for various nano- and analytical applications.
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