Microbes support their growth in vertebrate hosts by exploiting a large variety of dietary components as nutrients, which determines the composition of gut microbiota. A pathogen expands by utilizing 1,2-propanediol, a microbiota-fermented product, during mucosal inflammation. However, it remains largely unknown how the pathogen decides which nutrient to consume from the complex mixture in the gut. Here, we show that serovar Typhimurium utilizes 1,2-propanediol by EIIA (a nitrogen-metabolic PTS component implicated in virulence)-mediated regulation of the operon, thereby expanding in the murine intestine. Propionyl-CoA, a metabolic intermediate produced by 1,2-propanediol catabolism, elevates EIIA protein amounts, entailing positive feedback, thereby boosting the 1,2-propanediol-utilization process. EIIA promotes expression by enhancing glutathione synthesis. CRP (cAMP receptor protein) induces genes by increasing EIIA expression in response to glucose availability. Notably, EIIA-mediated 1,2-propanediol-utilization conferred a growth benefit even under high glucose conditions which reduces CRP activity. The EIIA-mediated activation is likely conserved in pathogenic enterobacteria including Collectively, our findings suggest that promotes its fitness by precisely modulating the utilization system for microbiota-derived carbon source. They also suggest that may integrate signals, processed via EIIA, into its metabolic program as well as virulence circuit.
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http://dx.doi.org/10.1021/acsinfecdis.0c00836 | DOI Listing |
Metabolites
November 2024
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Japan.
: Untargeted lipidomics using collision-induced dissociation-based tandem mass spectrometry (CID-MS/MS) is essential for biological and clinical applications. However, annotation confidence still relies on manual curation by analytical chemists, despite the development of various software tools for automatic spectral processing based on rule-based fragment annotations. : In this study, we present a novel machine learning model, MS2Lipid, for the prediction of known lipid subclasses from MS/MS queries, providing an orthogonal approach to existing lipidomics software programs in determining the lipid subclass of ion features.
View Article and Find Full Text PDFMicrobiol Spectr
November 2024
Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
Microbiome
May 2024
Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences, Soochow University, 199 Ren-Ai Road, 1132 Yunxuan Bldg, Suzhou, 215123, China.
Background: Gut microbiome metabolites are important modulators of host health and disease. However, the overall metabolic potential of the gut microbiome and interactions with the host organs have been underexplored.
Results: Using stable isotope resolved metabolomics (SIRM) in mice orally gavaged with C-inulin (a tracer), we first observed dynamic enrichment of C-metabolites in cecum contents in the amino acids and short-chain fatty acid metabolism pathways.
Mol Nutr Food Res
April 2024
School of Sport Science, Beijing Sport University, Beijing, 100084, China.
Scope: Urolithin A (UA), a gut-microbiota-derived metabolite of ellagic acid, presents various benefits to intestinal microecology. The presence of "gut-muscle axis" regulating the onset and progression of exercise-related physical frailty and sarcopenia has been recently hypothesized. This study aims to explore the underlying mechanism of gut-muscle axis by which UA enhances muscle strength and fatigue resistance of sleep-deprived (SD) mice.
View Article and Find Full Text PDFMicrobiome
March 2024
Division of Immunology, Otto Loewi Research Center for Vascular Biology, Immunology and Inflammation, Medical University of Graz, Stiftingtalstraße 6, 8010, Graz, Austria.
Background: Aronia melanocarpa is a berry rich in polyphenols known for health benefits. However, the bioavailability of polyphenols has been questioned, and the individual taste acceptance of the fruit with its specific flavor varies. We recently observed substantial differences in the tolerability of aronia juice among healthy females, with half of the individuals tolerating aronia juice without complaints.
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