Butanol fraction of De Wild. leaves ameliorate oxidative stress and modulate key hypoglycaemic processes in diabetic rats.

Objective: The effect of fractions on glucose homeostasis was investigated via enzyme inhibition activity, glucose uptake assay, and methods in diabetic rats.

Methodology: fractions were subjected to α-glucosidase inhibitory assay and then glucose uptake activity. The butanol fraction of the leaves (ABBF) was picked for the assay since it showed more activity in the initial tests conducted. ABBF was administrated via oral dosing to six-weeks old fructose-fed STZ-induced type 2 diabetic rats over a 5-week experimental period.

Results: ABBF treatment at a low dose of 150 mg/kg bw, significantly ( < .05) reduced blood glucose level, enhanced oral glucose tolerance ability, restored insulin secretion and hepatic glycogen synthesis as well as promoted islet regeneration than the high dose (300 mg/kg bw).

Conclusion: These results suggest that ABBF could be exploited as a therapeutic potential for treating T2D.