AI Article Synopsis
- Trinucleotide repeat sequences (TRSs) are common DNA microsatellites that vary in length and are linked to certain hereditary neurological diseases.
- The study explored how TRSs affect nucleosome formation using yeast minichromosomes, revealing that different TRS classes have distinct roles in promoting or excluding nucleosomes.
- Findings indicated that most TRSs, except for (AGG), influence nucleosome formation primarily through their binding affinity to histone octamers, offering insights into their behavior in the genome.
Article Abstract
Trinucleotide repeat sequences (TRSs), consisting of 10 unique classes of repeats in DNA, are members of microsatellites and abundantly and non-randomly distributed in many eukaryotic genomes. The lengths of TRSs are mutable, and the expansions of several TRSs are implicated in hereditary neurological diseases. However, the underlying causes of the biased distribution and the dynamic properties of TRSs in the genome remain elusive. Here, we examined the effects of TRSs on nucleosome formation in vivo by histone H4-S47C site-directed chemical cleavages, using well-defined yeast minichromosomes in which each of the ten TRS classes resided in the central region of a positioned nucleosome. We showed that (AAT) and (ACT) act as strong nucleosome-promoting sequences, while (AGG) and (CCG) act as nucleosome-excluding sequences in vivo. The local histone binding affinity scores support the idea that nucleosome formation in TRSs, except for (AGG), is mainly determined by the affinity for the histone octamers. Overall, our study presents a framework for understanding the nucleosome-forming abilities of TRSs.
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| http://dx.doi.org/10.1016/j.bbrc.2021.03.155 | DOI Listing |
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