Algal polysaccharide activates macrophages to alter physiologic biomarkers to drive the immunomodulatory phenotype, but it lacks specific biomarkers involved in the biochemical underpinning process. Here, we undertook an extensive analysis of the RAW 264.7 macrophages induced by an immunostimulating sulfated polysaccharide from Caulerpa racemosa var. peltata (CRVP-1) employing combined transcriptomic, proteomic, and metabolomic analyses to reveal the molecular details occurring in the CRVP-1-induced immunomodulatory process. The omics profiling of CRVP-1-activated macrophage demonstrated a total of 8844 genes (4354 downregulated and 4490 upregulated), 1243 proteins (620 downregulated and 623 upregulated), and 68 metabolites (52 downregulated and16 upregulated). Further, the co-mapped correlation network of omics combined with Western blot and immunofluorescence staining indicated that the cluster of differentiation 14 (CD14) might assist Toll-like receptor 4 (TLR4) involved in nuclear factor kappa-B (NF-κB) signaling pathway to drive the immunomodulatory phenotype. Together, our results discover novel physiologic biomarkers in the immunomodulatory activities of algal polysaccharides.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.04.025 | DOI Listing |
Sci Rep
November 2024
Department of Biological Sciences, Faculty of Sciences and Technology, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga), Yogyakarta, 55281, Indonesia.
Human melanoma is linked with aging-related disorders, prompting interest in the development of functional foods derived from natural ingredients to mitigate its incidence. Molecules in green seaweeds such as Caulerpa racemosa can serve this purpose due to their anti-tumor and anti-inflammatory properties. A previous work study compounds profiling has been carried out, and in this research the molecular docking studies targeting receptors associated with melanoma (GRP78, IRE1, BRAF) and aging (mTOR, AMPK, SIRT1) identified four promising compound in an extract of C.
View Article and Find Full Text PDFChemosphere
November 2024
Global Centre for Environmental Remediation (GCER), College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia.
Phytoremediation is a promising technology for treating Palm Oil Mill Effluent (POME). Moreover, phytoremediators have the potential for various aplication, including as feedstock. Hence, this study aims to elucidate the ability of sea grapes (Caulerpa racemosa) in remediating POME and evaluate their suitability as ornamental fish feed.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
School of Natural Sciences and Health, Tallinn University, Narva mnt 29, 10120 Tallinn, Estonia. Electronic address:
This research work explores the physicochemical characteristics and biological functions of polysaccharides extracted from four selected green seaweed species: Ulva lactuca (UL), Halimeda opuntia (HO), Caulerpa racemosa (CR), and Chaetomorpha antennina (CA). The extracts were investigated for cell-based bio-activities (i.e.
View Article and Find Full Text PDFMolecules
August 2024
Department of Cell and Molecular Biology, Biotechnology Center, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil.
Marine organisms represent a potential source of secondary metabolites with various therapeutic properties. However, the pharmaceutical industry still needs to explore the algological resource. The species Forssk presents confirmed biological activities associated with its major compound caulerpin, such as antinociceptive, spasmolytic, antiviral, antimicrobial, insecticidal, and cytotoxic.
View Article and Find Full Text PDFMar Drugs
June 2024
Department of Pathology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
The marine environment provides a rich source of distinct creatures containing potentially revolutionary bioactive chemicals. One of these organisms is , a type of green algae known as green seaweed, seagrapes, or green caviar. This organism stands out because it has great promise for use in medicine, especially in the study of cancer.
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