AI Article Synopsis
- The study involved analyzing a large number of bacterial genomes and discovered 829 gene clusters responsible for producing cyclodipeptides, specifically those that include a cytochrome P450 gene.
- Heterologous expression of a specific gene cluster resulted in the discovery of two new compounds, cyctetryptomycin A and B, which have unique macrocyclic structures and show potential neuroprotective effects.
- The identified cytochrome P450 enzymes facilitate a series of reactions that lead to the formation of these cyclic compounds from diketopiperazine precursors.
Article Abstract
We conducted global genome mining of 162,672 bacterial genomes and identified 829 cyclodipeptide (CDP) biosynthesis gene clusters (BGC) containing a cytochrome P450 gene. Heterologous expression of BGC from DSM 44795 led to the identification of two novel crownlike CDPs, cyctetryptomycin A () and B (), which possess unprecedented complex macrocycle and show neuroprotective activity. The two cytochrome P450s found in the BGC catalyze sequential reactions leading to the cyclization of diketopiperazine dimers.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.orglett.1c01022 | DOI Listing |
Publication Analysis
Top Keywords
global genome
8
genome mining
8
neuroprotective activity
8
mining reveals
4
reveals cytochrome
4
cytochrome p450-catalyzed
4
p450-catalyzed cyclization
4
cyclization crownlike
4
crownlike cyclodipeptides
4
cyclodipeptides neuroprotective
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!