In Colombia, identification of entomopathogenic nematodes (EPN's) native species is of great importance for pest management programs. The aim of this study was to isolate and identify EPNs and their bacterial symbiont in the department of Cauca-Colombia and then evaluate the susceptibility of two Hass avocado () pests to the EPNs isolated. EPNs were isolated from soil samples by the insect baiting technique. Their bacterial symbiont was isolated from hemolymph of infected larvae. Both organisms were molecularly identified. Morphological, and biochemical characterization was done for the bacteria. Susceptibility of and adults was evaluated by individually exposing adults to 50 infective juveniles. EPNs were allegedly detected at two sampled sites (natural forest and coffee cultivation) in 5.8% of the samples analyzed. However, only natural forest EPN's could be isolated and multiplied. The isolate was identified as BPS and its bacterial symbiont as BPS. Adults of both pests were susceptible to indicating this EPN potential for its management. The results of this study constitute the first record of in Colombia and the susceptibility of to this EPN.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015356 | PMC |
| http://dx.doi.org/10.21307/jofnem-2020-089 | DOI Listing |
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