Effect of Non-hydrogen Peroxide on Antibacterial Activity of Malaysian Honey against .

Introduction: Stingless bee is an insect that belongs to the family Apidae. Its name is based on its disability of stinging. It has a high product of honey and propolis by which are commonly referred to as stingless bee honey and stingless bee propolis. honey is one of the crucial natural sources and has the potential to kill infectious microorganisms. Previous studies have proved that the antibacterial activity of natural honey was an effect of hydrogen peroxide, a substance contained in the honey. However, these claims were contradicting with too many studies.

Objective: Therefore, this study aimed to identify the antibacterial activity of Malaysian honey which contained non-hydrogen peroxide against , an opportunistic microbial.

Materials And Methods: honey was used as an antibacterial agent for the treatment of in agar well diffusion assay. An amplex red hydrogen peroxide kit was used to identify the hydrogen peroxide in the honey sample. Meanwhile, non-hydrogen peroxide activity was performed by using honey-catalase treated.

Results: For the first time, we found that hydrogen peroxide was absent in all honey samples. honey has higher antibacterial activity (13.30 ± 0.56mm) compared to honey (9.03 ± 0.22mm) in agar well diffusion assay.

Discussion: Non-hydrogen peroxide in honey is a bioactive compound and beneficial to kill the microbial infection.

Conclusion: Antibacterial activity of Malaysian honey is directly contributed by non-hydrogen peroxide.