AI Article Synopsis
- The study investigates the oral and pulmonary mycobiomes in COPD patients and controls, revealing that the dominant fungus is Candida in both groups, with more found in oral samples than in lung samples.
- The research involved collecting samples from 93 COPD participants and 100 controls, analyzing fungal DNA, and comparing various metrics of fungal composition and diversity.
- Although no significant differences in fungal diversity or abundance were found between COPD and control groups, oral and pulmonary mycobiomes differed in composition, suggesting a distinct pulmonary mycobiome might exist.
Article Abstract
Background: The fungal part of the pulmonary microbiome (mycobiome) is understudied. We report the composition of the oral and pulmonary mycobiome in participants with COPD compared to controls in a large-scale single-centre bronchoscopy study (MicroCOPD).
Methods: Oral wash and bronchoalveolar lavage (BAL) was collected from 93 participants with COPD and 100 controls. Fungal DNA was extracted before sequencing of the internal transcribed spacer 1 (ITS1) region of the fungal ribosomal RNA gene cluster. Taxonomic barplots were generated, and we compared taxonomic composition, Shannon index, and beta diversity between study groups, and by use of inhaled steroids.
Results: The oral and pulmonary mycobiomes from controls and participants with COPD were dominated by Candida, and there were more Candida in oral samples compared to BAL for both study groups. Malassezia and Sarocladium were also frequently found in pulmonary samples. No consistent differences were found between study groups in terms of differential abundance/distribution. Alpha and beta diversity did not differ between study groups in pulmonary samples, but beta diversity varied with sample type. The mycobiomes did not seem to be affected by use of inhaled steroids.
Conclusion: Oral and pulmonary samples differed in taxonomic composition and diversity, possibly indicating the existence of a pulmonary mycobiome.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026037 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248967 | PLOS |
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