Objectives: To reconstruct the evolutionary history and genomic epidemiology of Staphylococcus aureus ST9 in China.
Methods: Using WGS analysis, we described the phylogeny of 131 S. aureus ST9 isolates collected between 2002 and 2016 from 11 provinces in China, including six clinical samples from Taiwan. We also investigated the complex structure and distribution of the lsa(E)-carrying multiresistance gene cluster, and genotyped prophages in the genomes of the ST9 isolates.
Results: ST9 was subdivided into one major (n = 122) and one minor (n = 9) clade. Bayesian phylogeny predicted the divergence of ST9 isolates in pig farming in China as early as 1987, which then evolved rapidly in the following three decades. ST9 isolates shared similar multiresistance properties, which were likely acquired before the ST9 emergence in China. The accessory genome is highly conserved, and ST9 harboured similar sets of phages, but lacked certain virulence genes.
Conclusions: Host exchange and regional transmission of ST9 have occurred between pigs and humans. Pig rearing and trading might have favoured gene exchanges between ST9 isolates. Resistance genes, obtained from the environment and other isolates, were stably integrated into the chromosomal DNA. The abundance of resistance genes among ST9 is likely attributed to the extensive use of antimicrobial agents in livestock. Phages are present in the genomes of ST9 and may play a role in the rapid evolution of this ST. Although human ST9 infections are rare, ST9 isolates may constitute a potential risk to public health as a repository of antimicrobial resistance genes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/jac/dkab106 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multilocus Sequence Typing and Antimicrobial Susceptibility of Isolated from Foods Surveyed in Kosovo.
Microorganisms
November 2024
Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa.
In the absence of data on the reporting of resistance to antibiotics, we sought to determine which clonal complexes (CCs)/sequence types (STs) circulate in the food chain in Kosovo and to determine their antibiogram profiles to a panel of 18 antibiotics. From a total of 114 isolates, 21 different typical STs were identified by multilocus sequence typing (MLST). Each isolate derived from the food categories was subjected to tests to verify its susceptibility to the selected antibiotics according to the designed Sensititre GPN3F panel.
View Article and Find Full Text PDFMolecular identification and subtypes analysis of Blastocystis in South Khorasan province, Eastern Iran.
Mol Biol Rep
December 2024
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background: Blastocystis is a prevalent intestinal parasitic protist that infects both birds and animals. There are at least 44 subtypes (ST) of Blastocystis, with ST1-ST9 being found in humans. The correlation between specific subtypes and pathogenicity has not been definitively established.
View Article and Find Full Text PDFAnalysis of Genetic Determinants Encoding Resistance to Heavy Metals and Disinfectants in .
Foods
December 2024
Department of Food Safety, National Institute of Public Health NIH-National Research Institute, 24 Chocimska str, 00-791 Warsaw, Poland.
is an important foodborne pathogen causing listeriosis. , existing in the natural environment, can also contaminate food products, which poses a serious threat to human health and life, especially for high-risk groups: pregnant women, newborn babies, and the elderly. Environmental adaptation of refers to the various strategies and mechanisms used by this bacterium to survive and thrive in diverse and often hostile environments that include, among others, toxic heavy metals and disinfectants.
View Article and Find Full Text PDFGenomic Insights into Antibiotic Resistance and Virulence of Isolated from Chongqing, China.
Foodborne Pathog Dis
December 2024
Chongqing Center for Disease Control and Prevention, Chongqing, China.
() is a pathogen of significant concern in food due to its ability to survive and multiply under harsh environmental conditions, such as high osmotic pressure, low temperatures, and freezing. This bacterium can cause listeriosis, a severe infection particularly dangerous for high-risk groups including newborns, pregnant women, and immunocompromised patients, due to its high morbidity and mortality rates. This study aimed to investigate the molecular epidemiological characteristics of isolated in Chongqing, southwest China.
View Article and Find Full Text PDFPrevalence and molecular epidemiology of methicillin-resistant Staphylococcus aureus in livestock farmers, livestock, and livestock products in southern Sri Lanka: A one health approach.
Infect Genet Evol
December 2024
Duke-Ruhuna Collaborative Research Centre, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka; Duke Global Health Institute, Durham, NC, USA; Department of Microbiology, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka.
Article Synopsis
- The study investigates MRSA prevalence and molecular epidemiology in livestock farmers, animals, and livestock products in southern Sri Lanka, using a One Health approach that connects human, animal, and environmental health.
- It found a low MRSA colonization prevalence in the region, with 24.0% in farms and 7.9% in farmers, while identifying two new MRSA clones in human and animal samples, but no livestock-associated MRSA (LA-MRSA) clones.
- The research underscores the need for ongoing MRSA surveillance to track transmission dynamics between humans and animals within the One Health framework.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!