Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8544647 | PMC |
| http://dx.doi.org/10.1038/s41593-021-00823-7 | DOI Listing |
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