AI Article Synopsis
- Leaf rust, caused by a specific fungus, threatens wheat production by diminishing both the quantity and quality of cereal yields.
- The research focused on identifying markers linked to slow rust genes for developing wheat cultivars with stable horizontal-type resistance, which is more effective than major resistance genes that pathogens can overcome.
- The study successfully utilized multiplex PCR techniques and SSR-PCR markers to identify resistant wheat lines, particularly PHR 4947 and PHR 4819, proving that marker-assisted selection is effective for breeding rust-resistant crops.
Article Abstract
Leaf rust caused by the fungus f. sp. is one of the most dangerous diseases of common wheat. Infections caused by fungal pathogens reduce the quantity and quality of yields of many cereal species. The most effective method to limit plant infection is to use cultivars that show rust resistance. Genetically conditioned horizontal-type resistance (racial-nonspecific) is a desirable trait because it is characterized by more stable expression compared to major (R) genes that induce racially specific resistance, often overcome by pathogens. Horizontal resistance is conditioned by the presence of slow rust genes, which include genes and . This study aimed to identify markers linked to both genes in 64 common wheat lines and to develop multiplex PCR reaction conditions that were applied to identify both genes simultaneously. The degree of infestation of the analyzed lines was also assessed in field conditions during the growing season of 2017 and 2018. Simple sequence repeat anchored-polymerase chain reaction (SSR-PCR) marker was identified during analysis in line PHR 4947. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. In addition to gene , gene was identified in this genotype. Lines PHR 4947 and PHR 4819 were characterized by the highest leaf rust resistance in field conditions. During STS-PCR analyses, the marker of gene was identified in most of the analyzed lines. This marker was not present in the following genotypes: PHR 4670, PHR 4800, PHR 4859, PHR 4907, PHR 4922, PHR 4949, PHR 4957, PHR 4995, and PHR 4997. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. Genotypes carrying the markers of the analyzed gene showed good resistance to leaf rust in field conditions in both 2017 and 2018. Research has demonstrated that marker assisted selection (MAS) and multiplex PCR techniques are excellent tools for selecting genotypes resistant to leaf rust.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968542 | PMC |
| http://dx.doi.org/10.1515/biol-2021-0018 | DOI Listing |
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