has been used for centuries in China and its polysaccharides are the main active components in treating loss of body fluids resulting from fever and asthenic symptoms. However, the biosynthetic pathway of polysaccharides in remains to be elucidated. In this study, we obtained a guanosine diphosphate (GDP)-mannose pyrophosphorylase (DhGMPP) from and characterized its function to catalyze the conversion of α-D-mannose-phosphate to GDP-D-mannose involved in the production of polysaccharides. , with the open reading frame of 1,245 bp, was isolated from RNA-Seq data of . Phylogenetic analysis as well as sequence characterization suggested its involvement in the biosynthesis of GDP-D-mannose. enzyme assay demonstrated that encoded a pyrophosphorylase that converted -D-mannose-phosphate and GTP into GDP-D-mannose. Identification of could provide more insights into the mechanism concerning polysaccharide biosynthesis in and be utilized for enhancing polysaccharide accumulation through metabolic engineering.
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| http://dx.doi.org/10.1515/biol-2021-0015 | DOI Listing |
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