The effectiveness of genes is known to be resistant to downy mildew (DM) disease affected by fungus in sunflower. In this study phenotypic analysis was performed using inoculation tests and genotypic analysis were carried out with three DM resistance genes and . A total of 69 simple sequence repeat markers and 241 F individuals derived from a cross of RHA-419 (R) x P6LC (S), RHA-419 (R) x CL (S), RHA-419 (R) x OL (S), RHA419 (R) x 9758R (S), HA-R5 (R) x P6LC (S) and HA89 (R) x P6LC (S) parental lines were used to identify resistant hybrids in sunflower. Results of SSR analysis using markers linked with downy mildew resistance genes (, and ) and downy mildew inoculation tests were evaluated together and and (for ) markers showed positive correlation with their phenotypic results. These results suggest that these markers are associated with DM resistance and they can be used successfully in marker-assisted selection for sunflower breeding programs specific for downy mildew resistance.
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http://dx.doi.org/10.1515/biol-2018-0039 | DOI Listing |
Methods Mol Biol
December 2024
United States Department of Agriculture, Agricultural Research Service, Foreign Disease/Weed Science Research Unit, Frederick, MD, USA.
Fluorescence in situ hybridization enables the visualization of organisms in the environment without having to culture them. Here, we describe a FISH protocol to visualize oomycete structures (mycelia, sporangiophores, sporangia, and oospores) directly as well as from colonized plant material. The protocol utilizes organic compounds with low toxicities and does not require a permeabilization step.
View Article and Find Full Text PDFBMC Plant Biol
December 2024
University of Sciences and Art Western Switzerland, Changins College for Viticulture and Enology, Route de Duillier 50, Nyon, 1260, Switzerland.
Background: Priming plants with natural products is extensively studied in the agricultural field to reduce the use of synthetic and copper-based pesticides. Previous studies have shown that Oregano essential oil vapour (OEOV) is an effective priming agent against downy mildew (DM) in grapevine (Vitis vinifera L. cv.
View Article and Find Full Text PDFEcotoxicol Environ Saf
December 2024
Center for Clinical Medicine Research, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China. Electronic address:
Dimethomorph (DMT) is a widely-used selective active fungicide that effectively controls downy mildew, crown rot, and late blight in crops. The extensive application of DMT raises concerns about its ecological impact on non-target organisms in the environment. However, there is limited understanding of the toxicological properties of DMT on these organisms.
View Article and Find Full Text PDFInt J Mol Sci
November 2024
Department of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences-SGGW, 159 Nowoursynowska Str., 02-776 Warsaw, Poland.
Cucurbit downy mildew, caused by , is a devastating disease in cucumbers that leads to significant yield losses in many cucurbit-growing regions worldwide. Developing resistant cucumber varieties is a sustainable approach to managing this disease, especially given the limitations of chemical control and the evolving nature of pathogens. This article reviews the genetic basis of downy mildew resistance in cucumbers, emphasizing key resistance (R) genes and quantitative trait loci (QTLs) that have been mapped.
View Article and Find Full Text PDFPlants (Basel)
November 2024
Department of Plant Pathology, Faculty of Agriculture, Ain Shams University, Cairo 11241, Egypt.
Quinoa ( Willd.) is a new, promising non-conventional useful crop; however, its susceptibility to downy mildew, caused by , is a key obstacle limiting its productivity in Egypt. Identifying and utilizing resistant quinoa cultivars appear to be reliable and cost-efficient strategies for controlling downy mildew, particularly in resource-limited farmers' fields.
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