Background: Wound healing is one of the defining features of life and is seen not only in tissues but also within individual cells. Understanding wound response at the single-cell level is critical for determining fundamental cellular functions needed for cell repair and survival. This understanding could also enable the engineering of single-cell wound repair strategies in emerging synthetic cell research. One approach is to examine and adapt self-repair mechanisms from a living system that already demonstrates robust capacity to heal from large wounds. Towards this end, Stentor coeruleus, a single-celled free-living ciliate protozoan, is a unique model because of its robust wound healing capacity. This capacity allows one to perturb the wounding conditions and measure their effect on the repair process without immediately causing cell death, thereby providing a robust platform for probing the self-repair mechanism.
Results: Here we used a microfluidic guillotine and a fluorescence-based assay to probe the timescales of wound repair and of mechanical modes of wound response in Stentor. We found that Stentor requires ~ 100-1000 s to close bisection wounds, depending on the severity of the wound. This corresponds to a healing rate of ~ 8-80 μm/s, faster than most other single cells reported in the literature. Further, we characterized three distinct mechanical modes of wound response in Stentor: contraction, cytoplasm retrieval, and twisting/pulling. Using chemical perturbations, active cilia were found to be important for only the twisting/pulling mode. Contraction of myonemes, a major contractile fiber in Stentor, was surprisingly not important for the contraction mode and was of low importance for the others.
Conclusions: While events local to the wound site have been the focus of many single-cell wound repair studies, our results suggest that large-scale mechanical behaviors may be of greater importance to single-cell wound repair than previously thought. The work here advances our understanding of the wound response in Stentor and will lay the foundation for further investigations into the underlying components and molecular mechanisms involved.
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http://dx.doi.org/10.1186/s12915-021-00970-0 | DOI Listing |
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January 2025
Department of Arthroscopic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, No. 600 Yishan Road, Xuhui District, Shanghai 200233, China.
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January 2025
Department of Emergency Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.
Introduction: Bacterial infection, a complex wound microenvironment, and a persistent inflammatory response in acute wounds can result in delayed healing and abnormal scar formation, thereby compromising the normal function and aesthetic appearance of skin tissue. This issue represents one of the most challenging problems in clinical practice. This study aims to develop a hydrogel dressing specifically designed for the treatment of acute wounds, providing immediate and effective protection for the affected areas.
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January 2025
Department of Health Sciences, Lund University, Lund, Sweden.
Epidemiological studies on elite female ice hockey players are lacking but needed to tailor preventive efforts in this growing group of athletes. Therefore, the aim of this study was to describe the incidence, prevalence, and burden of health problems in elite female ice hockey players. In this prospective cohort study, we asked all Swedish Women's Hockey League (SWHL) players ( = 207) to report their health status on the OSTRC-H2 weekly throughout the 2022/2023 season (28 weeks).
View Article and Find Full Text PDFHeliyon
January 2025
Anhui Medical University School of Basic Medicine, Hefei, 230032, China.
The anxieties and concerns about health hazards caused by microwave has been growing recently. Previous studies have reported microwave induced structural and functional injuries to brain. However, the biological effects caused by compound microwave were largely unexplored.
View Article and Find Full Text PDFNew Solut
January 2025
South Asian Women and Immigrants' Services, Toronto, Ontario, Canada.
Employers sometimes hinder the appropriate reporting of claims to workers' compensation, a phenomenon termed claim suppression. While the magnitude of claim suppression is difficult to quantify, various reports have identified it as a significant concern. In response, several Canadian jurisdictions, such as Ontario in 2015, introduced legislation addressing claim suppression.
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