AI Article Synopsis
- DNA methylation is a common regulatory modification in both prokaryotes and eukaryotes, and mA modifications can alter DNA structures.
- The study examines how mA and mA modifications affect the structure and stability of a DNA minidumbbell formed by TTTA tetranucleotide repeats.
- Results from nuclear magnetic resonance studies show that mA promotes the formation of specific base pairs while mA has a destabilizing effect, offering new insights into how adenine methylation influences DNA stability.
Article Abstract
DNA methylation is a prevalent regulatory modification in prokaryotes and eukaryotes. -methyladenine (mA) and -methyladenine (mA) have been found to be capable of altering DNA structures via disturbing Watson-Crick base pairing. However, little has been known about their influences on non-B DNA structures, which are associated with genetic instabilities. In this work, we investigated the effects of mA and mA on both the structure and thermodynamic stability of a newly reported DNA minidumbbell formed by two TTTA tetranucleotide repeats. As revealed by the results of nuclear magnetic resonance spectroscopic studies, both mA and mA favored the formation of a T·mA and T·mA Hoogsteen base pair, respectively. More intriguingly, the mA and mA modifications brought about stabilization and destabilization effects on the DNA minidumbbell, respectively. This work provides new biophysical insights into the effects of adenine methylation on the structure and thermodynamic stability of DNA.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037738 | PMC |
| http://dx.doi.org/10.3390/ijms22073633 | DOI Listing |
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