In a recent monograph on the genus , type specimens worldwide were revised and re-classified using a morphological approach. Among them, some came from Pier Andrea Saccardo's fungarium stored in the Herbarium of the Padova Botanical Garden. In this work, we taxonomically re-examine via a morphological and molecular approach nine different Saccardo types. ITS1 and/or ITS2 sequences were successfully obtained applying Illumina MiSeq technology and phylogenetic analyses were carried out in order to elucidate their current taxonomic position. Only the ITS1 sequence was recovered for , while for , only the ITS2 sequence was recovered. We proposed here new combinations for , and , while for , , , and , we did not suggest taxonomic changes compared to the current ones. The name Sacc. is invalid, as it is a later homonym of P. Karst. & Starbäck. Therefore, we introduced as a nomen novum for Sacc. This is the first time that these types have been subjected to a molecular study. Our results demonstrate that old types are an important source of DNA sequence data for taxonomic re-examinations.
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http://dx.doi.org/10.3390/microorganisms9030666 | DOI Listing |
Since late 2021, a panzootic of highly pathogenic H5N1 avian influenza virus has driven significant morbidity and mortality in wild birds, domestic poultry, and mammals. In North America, infections in novel avian and mammalian species suggest the potential for changing ecology and establishment of new animal reservoirs. Outbreaks among domestic birds have persisted despite aggressive culling, necessitating a re-examination of how these outbreaks were sparked and maintained.
View Article and Find Full Text PDFPlant Dis
January 2025
University of Florida Tropical Research and Education Center, Plant Pathology, 1615 SE 23rd Way, Homestead, Florida, United States, 33031-3314;
The commercial production of passion fruit is geographically limited (California, Florida, and Hawaii), but the development of cold-tolerant varieties could expand it beyond warm-climate states (Stafne et.al. 2023).
View Article and Find Full Text PDFJ Transl Med
January 2025
Tumour Biology and Immunology Laboratory, Research Branch, Sidra Medicine, Doha, Qatar.
Background: FFPE tissue samples are commonly used in biomedical research and are a valuable source for next-generation sequencing in oncology, however, extracting RNA from these samples can be difficult the quantity and quality achieved can impact the downstream analysis. This study compared the effectiveness of seven different commercially available RNA extraction kits specifically designed for use with FFPE samples in terms of the quantity and quality of RNA recovered.
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BMC Infect Dis
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Department of Microbiology and Infectious Diseases, Nara Medical University, Shijo-cho, Kashihara, Nara, Japan.
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Genetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON M5G0A4, Canada.
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