Biofilm-Forming Ability of and Considering Physicochemical and Topographical Surface Properties.

Biofilm characteristics of D84 () and subsp. () on polytetrafluoroethylene and AISI-304 stainless steel at early- (24, 48 h) and late-stage (144, 192 h) biofilm formation were investigated. biofilm structure was more developed compared to , representing vastly mature biofilms with a strongly developed amorphous matrix, possibly extracellular polymeric substances (EPSs), at late-stage biofilm formation. showed faster growth behavior but still resulted in a relatively flat biofilm structure. Strong correlations were found between several roughness parameters and surface coverage (r ≥ 0.98), and between total surface free energy (γs) and surface coverage (r = 0.89), while remained mostly unaffected. The pronounced ubiquitous biofilm characteristics make D84 a suitable model for biofilm research. Studying biofilm formation of these bacteria may help one understand bacterial adhesion on interfaces and hence reduce biofilm formation in the food industry.