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Comparison of Two Transmission Electron Microscopy Methods to Visualize Drug-Induced Alterations of Gram-Negative Bacterial Morphology. | LitMetric

Comparison of Two Transmission Electron Microscopy Methods to Visualize Drug-Induced Alterations of Gram-Negative Bacterial Morphology.

Antibiotics (Basel)

Australia Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, Roseworthy Campus, The University of Adelaide, Roseworthy, SA 5371, Australia.

Published: March 2021

AI Article Synopsis

  • The study optimized and compared transmission electron microscopy (TEM) methods for observing Gram-negative bacteria treated with a combination of robenidine analogue (NCL195) and colistin.
  • Aldehyde-fixed bacterial samples were prepared using conventional TEM and compared to ultrathin Tokuyasu cryo-sections.
  • Results showed that ultrathin cryo-sections provided better visualization of bacterial membrane structures, helping to reveal how drugs interact with the bacterial cell membrane more clearly.

Article Abstract

In this study, we optimized and compared different transmission electron microscopy (TEM) methods to visualize changes to Gram-negative bacterial morphology induced by treatment with a robenidine analogue (NCL195) and colistin combination. Aldehyde-fixed bacterial cells (untreated, treated with colistin or NCL195 + colistin) were prepared using conventional TEM methods and compared with ultrathin Tokuyasu cryo-sections. The results of this study indicate superiority of ultrathin cryo-sections in visualizing the membrane ultrastructure of and , with a clear delineation of the outer and inner membrane as well as the peptidoglycan layer. We suggest that the use of ultrathin cryo-sectioning can be used to better visualize and understand drug interaction mechanisms on the bacterial cell membrane.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002630PMC
http://dx.doi.org/10.3390/antibiotics10030307DOI Listing

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