Coffee corky-root disease causes serious damages to coffee crop and is linked to combined infection of spp. and root-knot nematodes spp. In this study, 70 isolates were collected from both roots of healthy coffee plants and with corky-root disease symptoms. A phylogenetic analysis, and the detection of pathogenicity genes and toxigenicity genes was performed for 59 and 11 isolates. Based on the molecular characterization, seven and three isolates were assessed for their pathogenicity on coffee seedlings under optimal watering and water stress miming root-knot nematode effect on plants. Our results revealed that a drastic increment of plant colonization capacity and pathogenicity on coffee plants of some isolates was caused by water stress. The pathogenicity on coffee of linked to coffee corky-root disease and the presence of genes in this species were demonstrated for the first time. Our study provides evidence for understanding the pathogenic basis of and isolates on coffee and revealed the presence of and genes as one of their pathogenicity-related mechanisms. We also highlight the relevance of chlorophyll, a fluorescence as an early and high-throughput phenotyping tool in pathogenicity studies on coffee.
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| http://dx.doi.org/10.3390/jof7040253 | DOI Listing |
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Article Synopsis
- The study investigates the interaction of root-knot nematodes and phytopathogenic fungi in disease complexes affecting crops like tomato and coffee.
- By using 16s rDNA gene sequencing, researchers analyzed the bacterial communities in healthy and infested roots, as well as in the nematodes and their eggs.
- The findings revealed distinct bacterial taxonomic diversities for each crop, identifying specific bacterial orders that drive the infection process, while also predicting shifts in the roots' microbial metabolic profiles linked to disease development.
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