Characterization of the complete mitochondrial genome of the Northern Mud Gudgeon, (Perciformes: Eleotridae) with phylogenetic implications.

The first mitochondrial genome of was determined by the combination of next-generation sequencing (NGS) and Sanger sequencing methods. A complete circular mitogenome of (16,529 bp) consisted of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two non-coding regions, including a control region (D-loop) and a light strand origin of replication (O). Two start codons (ATG and GTG) and four stop codons (TAG, TAA, TA-, and T-) were used in all the PCGs. Except for ND6 and eight transfer RNAs (tRNAs), all the other genes were encoded in the heavy strand. Based on phylogenetic analysis, formed a clade with three other species in the subfamily Butinae, while the other 10 made a subfamily Eleotrinae clade.