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Fast, multiplane line-scan confocal microscopy using axially distributed slits. | LitMetric

Fast, multiplane line-scan confocal microscopy using axially distributed slits.

Biomed Opt Express

Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.

Published: March 2021

AI Article Synopsis

Article Abstract

The inherent constraints on resolution, speed and field of view have hindered the development of high-speed, three-dimensional microscopy techniques over large scales. Here, we present a multiplane line-scan imaging strategy, which uses a series of axially distributed reflecting slits to probe different depths within a sample volume. Our technique enables the simultaneous imaging of an optically sectioned image stack with a single camera at frame rates of hundreds of hertz, without the need for axial scanning. We demonstrate the applicability of our system to monitor fast dynamics in biological samples by performing calcium imaging of neuronal activity in mouse brains and voltage imaging of cardiomyocytes in cardiac samples.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984773PMC
http://dx.doi.org/10.1364/BOE.417286DOI Listing

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