AI Article Synopsis
- - Researchers developed new intestinal organoids from human iPS cells that better mimic mature small intestine structures, overcoming issues with previous methods.
- - These organoids exhibited high levels of key drug transporters and can imitate gut diseases when exposed to specific cytokines, allowing for a better understanding of conditions like mucosal damage and intestinal fibrosis.
- - The organoids maintained their intestinal properties and showed significant electrical resistance when cultured in a specific setup, indicating their potential for use in disease modeling and drug testing.
Article Abstract
Human induced pluripotent stem (iPS) cell-derived intestinal organoids have low invasiveness; however, the current differentiation method does not reflect the crypt-villus-like structure due to structural immaturity. Here, we generated budding-like organoids that formed epithelial tissue-like structures and had the characteristics of the mature small intestine from human iPS cells. They showed a high expression of drug transporters and induced the expression of cytochrome P450 3A4 and P-glycoprotein. When treated with tumor necrosis factor-α and/or transforming growth factor-β, the budding-like organoids replicated the pathogenesis of mucosal damage or intestinal fibrosis. Upon dissociation and seeding on cell culture inserts, the organoids retained intestinal characteristics, forming polarized intestinal folds with approximately 400 Ω × cm transepithelial electrical resistance. This novel method has great potential for disease modeling and drug screening applications.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.xphs.2021.03.014 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!