AI Article Synopsis
- This study focuses on creating an antiviral treatment using curcumin encapsulated in biodegradable nanoparticles made from poly(lactic-co-glycolic acid) (PLGA) to combat the Zika virus.
- The researchers characterized the PLGA-curcumin nanoparticles (PLGA-CUR NPs) through various scientific methods and tested their antiviral effects and cytotoxicity in Vero cells.
- The findings reveal that PLGA-CUR NPs are more effective than free curcumin at reducing Zika virus infection, indicating their potential as a drug formulation against harmful flaviviruses.
Article Abstract
Objectives: In the search of an effective antiviral formulation, the natural product curcumin (CUR) was encapsulated into poly(lactic-co-glycolic acid) nanoparticles, a non-toxic bioresorbable and biocompatible copolymer. The resulting CUR containing particles (PLGA-CUR NPs) were characterized and analysed for antiviral activity against Zika virus (ZIKV) infection.
Methods: The PLGA-CUR NPs were characterized by Fourier transform infrared, differential scanning calorimetry, dynamic light scattering, scanning electron microscopy and thermogravimetric analysis and release profile. Cytotoxicity of PLGA-CUR and the antiviral activity against ZIKV were determined in Vero cells. The effect of PLGA-CUR NPs on viral RNA synthesis and protein expression was analysed by RT-qPCR and immunofluorescence staining, respectively.
Key Findings: The PLGA-CUR NPs showed an appropriate in vitro drug release profile. Our studies of the antiviral activity of PLGA-CUR NPs and CUR against ZIKV by virus yield reduction as well as viral RNA synthesis and protein expression have shown that PLGA-CUR formulation is more effective than free CUR to inhibit ZIKV infection of Vero cells.
Conclusions: Our results demonstrate for the first time the antiviral activity against ZIKV of PLGA nanoparticles charged with CUR, suggesting that PLGA-CUR NPs are promising candidates for a drug formulation against human pathogenic flaviviruses.
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| http://dx.doi.org/10.1093/jpp/rgaa045 | DOI Listing |
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