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Improved Yield of Recombinant Protein via Flagella Regulator Deletion in . | LitMetric

Improved Yield of Recombinant Protein via Flagella Regulator Deletion in .

Front Microbiol

Department of Chemical and Biological Engineering, Korea University, Seoul, South Korea.

Published: March 2021

AI Article Synopsis

  • Protein production requires a lot of energy, and researchers aimed to improve this process by removing flagella to cut down on energy use.
  • The deletion of the FlhC gene, which controls flagella assembly, led to slower growth but increased ATP levels and a higher NADPH/NADP ratio in the modified strain.
  • Despite the growth reduction, introducing a plasmid or overexpressing a protein restored growth rates while improving the yield of a fluorescent protein by 1.81 times, indicating efficient protein production with less energy spent on flagella.

Article Abstract

Protein production requires a significant amount of intracellular energy. Eliminating the flagella has been proposed to help improve protein production by reducing energy consumption. In this study, the gene encoding a subunit of FlhC, a master regulator of flagella assembly, was deleted to reduce the expression of flagella-related genes. FlhC knockout in the -deleted strain triggered significant growth retardation with increased ATP levels and a higher NADPH/NADP ratio. Metabolic flux analysis using a C-labeled carbon substrate showed increased fluxes toward the pentose phosphate and tricarboxylic acid cycle pathways in the - and -deleted strains. Introduction of a high copy number plasmid or overexpression of the recombinant protein in this strain restored growth rate without increasing glucose consumption. These results suggest that the metabolic burden caused by deletion was resolved by recombinant protein production. The recombinant enhanced green fluorescent protein yield per glucose consumption increased 1.81-fold in the mutant strain. Thus, our study demonstrates that high-yield production of the recombinant protein was achieved with reduced flagella formation.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005581PMC
http://dx.doi.org/10.3389/fmicb.2021.655072DOI Listing

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