Molecular, Morphological, and Spatial Study of (Pulmonata, Lymnaeidae) from Southeastern Iran.

Background: Snails of the genus are the intermediate hosts of species the etiological agents of liver fluke disease, fascioliasis. A genetically different but morphologically very similar species in the genus, is sympatrically distributed with in some regions of the world. We aimed to investigate the occurrence of in Kerman province, Iran and to characterize genetically specimens from southeast Iran.

Methods: Field-collected snails from four localities in Jiroft, Bam and Faryab, Kerman province, southeastern Iran were studied. Hydrological variables including temperature and pH were recorded for each habitat. Each specimen was identified using morphological as well as conchological characteristics. Genetic characterization was performed using PCR-sequencing followed by phylogenetic analyses on nuclear as well as mitochondrial gene fragments. MaxEnt software was used to predict the most appropriate ecological niches for the targeted species.

Results: was found in 4 out of 28 locations. One and two haplotypes were detected among populations from the four localities. Habitat study showed that thrives in habitats with alkaline pH. from South America were clustered with specimens from Bam, Kerman, Iran; however, north Iranian isolates of were strongly correlated with specimens from Jiroft and Faryab. MaxEnt model for the most appropriate ecological niches of the targeted species predicted environmental suitability for this species in western Africa as well as coastal areas in north and southwestern Africa.

Conclusion: is frequently present in southern areas of Kerman Province. At least two genetically different haplotypes are present in southeastern Iran.