Marine algae (seaweed) encompass numerous groups of multicellular organisms with various shapes, sizes, and colors, and serve as important sources of natural bioactive substances. The brown alga Kjellman, an edible seaweed, contains many bioactives such as phlorotannins and fucoidans. Here, we evaluated the antioxidative, neuroprotective, and anti-apoptotic effects of extract (ECE), phlorotannin-rich extract (ECPE), and the phlorotannin dieckol on neuronal PC-12 cells. The antioxidant capacities of ECPE and ECE were 1,711.5 and 1,050.4 mg vitamin C equivalents/g in the ABTS assay and 704.0 and 474.6 mg vitamin C equivalents/g in the DPPH assay, respectively. The dieckol content of ECPE (58.99 mg/g) was approximately 60% higher than that of ECE (36.97 mg/g). Treatment of PC-12 cells with ECPE and ECE increased cell viability in a dose-dependent manner. Intracellular oxidative stress in PC-12 cells due to ECPE and ECE decreased dose-independently by up to 63% and 47%, respectively, compared with the stress control (323%). ECPE reduced the production of the pro-apoptotic proteins Bax and caspase-3 more effectively than ECE. Early and late apoptosis in PC-12 cells were more effectively decreased by ECPE than ECE treatments. From the results obtained in this study, we concluded that ECPE, which is rich in phlorotannins, including the marker compound dieckol, may be applied to the development of functional materials for improving cognition and memory.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705912 | PMC |
http://dx.doi.org/10.4014/jmb.2012.12013 | DOI Listing |
Eur J Med Chem
December 2024
Shaanxi Key Laboratory of Natural Products & Chemical Biology, Shaanxi Engineering Center of Bioresource Chemistry & Sustainable Utilization, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, 712100, PR China. Electronic address:
Eleven new chromane meroterpenoids (1-11), along with 24 known ones (12-35) were isolated from Rhododendron capitatum, a Tibetan medicine. Their structures were determined via extensive spectroscopic methods. The absolute configurations of 1 and 2 were determined by comparison of the experimental and theoretically calculated ECD data.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China. Electronic address:
To develop a scaffold suitable for simultaneous repair of both spinal cord injury (SCI) and sciatic nerve injury (SNI), we designed a multilayer composite membrane capable of unidirectional and sustained release of two factors: nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). The membrane's morphology, mechanical properties, cytocompatibility, drug release kinetics, swelling, and degradation behavior were thoroughly characterized. Additionally, its ability to promote the differentiation of PC-12 cells was assessed.
View Article and Find Full Text PDFJ Synchrotron Radiat
January 2025
School of Science, RMIT University, 2476, Melbourne, Victoria 3001, Australia.
Synchrotron sourced Fourier transform infrared (SS FTIR) microspectroscopy was employed to investigate the biological effects on the neuron-like pheochromocytoma (PC 12) cells after exposure to synchrotron sourced terahertz (SS THz) radiation. Over 10 min of exposure, the PC 12 cells received a total energy of 600 J m, with a total incident power density of ∼1.0 W m (0.
View Article and Find Full Text PDFSci Rep
December 2024
Department of Rehabilitation Medicine, the first Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.
Angew Chem Int Ed Engl
November 2024
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Nanofluidic memristors have recently been reshaped into artificial synapses capable of mimicking many fundamental neurosynaptic patterns, while sense digitalization has been increasingly explored to link the neuromorphic devices with external equipment. By inspiration of dopaminergic nerve, here a nanofluidic nerve with sense digitalization is devised by engineering a dopamine (DA)-specific nanofluidic synapse as mediated by PC-12 cells to manage the robotic arm. Different from previous neuromorphic perception of DA via redox reaction, the aptamer-based perception here is based on biological DA recognition by its receptor as indicated by the ionic signals.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!