Genetic Diversity of in Mexican Vineyards.

Plant Dis

Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. IPN 2508 San Pedro Zacatenco, 07360 CDMX, México.

Published: May 2021

AI Article Synopsis

  • A xylem-inhabiting bacterium, affecting key crops, has been found in areas of Mexico including Baja California, Coahuila, and Querétaro.
  • Researchers collected 408 grapevine samples to assess the genetic diversity of this bacterium, using techniques like real-time PCR and multilocus sequence typing.
  • The findings revealed that while isolates from Baja California and Coahuila were similar to those in the U.S., the Querétaro samples exhibited notable genetic differences, indicating diversity among the bacterium's populations in Mexico.

Article Abstract

is a xylem-inhabiting phytopathogenic bacterium that affects diverse agriculturally relevant crops. In Mexico, has been reported in the states of Baja California, Coahuila, and Querétaro. In order to determine the genetic diversity of this bacterium in Mexico, 408 grapevine samples were collected from the main producing states in México. For identification, real-time PCR and three-loci end-point PCR were employed. The genotyping of the subspecies was carried out using multilocus sequence typing and analysis, based on seven housekeeping genes: , and The resulting sequences were compared with those present in extant databases. The presence of subsp. in the states of Baja California (sequence type 1), Coahuila (sequence type 1), and Querétaro was confirmed. The isolates from northern Mexico bear high similarity to grapevine isolates from the United States. However, the isolates from Querétaro showed significant differences with currently known sequences, showing that there is genetic variability among the subsp. populations from grapevines in northern and central Mexico.

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Source
http://dx.doi.org/10.1094/PDIS-09-20-1900-REDOI Listing

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