Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca activities with different spatiotemporal patterns during migration. Examining the Ca activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca activity and morphology of growing brain ETCs in larval zebrafish. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).
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| http://dx.doi.org/10.1016/j.xpro.2021.100388 | DOI Listing |
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