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Entrainment of cerebellar Purkinje cell spiking activity using pulsed ultrasound stimulation. | LitMetric

Entrainment of cerebellar Purkinje cell spiking activity using pulsed ultrasound stimulation.

Brain Stimul

Department of Biomedical Engineering, New Jersey Institute of Technology, Newark, NJ, USA. Electronic address:

Published: November 2021

AI Article Synopsis

  • Focused ultrasound (FUS) is a non-invasive method with precise targeting abilities, but its effects on cerebellar Purkinje cells (PCs) remain unclear.
  • The study aimed to examine how different FUS pulse durations and repetition frequencies influence the activity of PCs in the cerebellum of anesthetized rats.
  • Results indicated that FUS can effectively modulate the spiking activity of PCs, showing stronger effects with shorter pulse durations and a potential combination of excitatory and inhibitory influences across the entire cortical network.

Article Abstract

Background: Focused ultrasound (FUS) has excellent characteristics over other non-invasive stimulation methods in terms of spatial resolution and steering capability of the target. FUS has not been tested in the cerebellar cortex and cellular effects of FUS are not fully understood.

Objective/hypothesis: To investigate how the activity of cerebellar Purkinje cells (PCs) is modulated by FUS with varying pulse durations and pulse repetition frequencies.

Methods: A glass microelectrode was inserted into the cerebellar vermis lobule 6 from the dorsal side to extracellularly record single unit activity of the PCs in anesthetized rats. Ultrasonic stimulation (500 kHz) was applied through a coupling cone, filled with degassed water, from the posterior side to target the recording area with varying pulse durations and frequencies.

Results: Simple spike (SS) activity of PCs was entrained by the FUS pattern where the probability of spike occurrences peaked at around 1 ms following the onset of the stimulus regardless of its duration (0.5, 1, or 2 ms). The level of entrainment was stronger with shorter pulse durations at 50-Hz pulse repetition frequency (PRF), however, peri-event histograms spread wider and the peaks delayed slightly at 100-Hz PRF, suggesting involvement of a long-lasting inhibitory mechanism. There was no significant difference between the average firing rates in the baseline and stimulation periods.

Conclusion: FUS can entrain spiking activity of single cells on a spike-by-spike basis as demonstrated here in the rat cerebellar cortex. The observed modulation potentially results from the aggregate of excitatory and inhibitory effects of FUS on the entire cortical network rather than on the PCs alone.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164992PMC
http://dx.doi.org/10.1016/j.brs.2021.03.004DOI Listing

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