AI Article Synopsis
- The study investigates oxidative RNA damage in patients with colorectal cancer (CRC) by measuring 8-hydroxyguanine (8-OHG) in urine and comparing it with healthy controls.
- Preoperative results show that CRC patients had significantly higher 8-OHG levels than healthy individuals, particularly those in stages II/III of the disease.
- After surgery, 8-OHG levels in CRC patients were similar to controls, suggesting oxidative damage is more pronounced before surgery and could serve as a marker for advanced disease stages.
Article Abstract
Purpose: Cellular RNA is less compact than DNA, more easily accessible to ROS and therefore could be more susceptible to oxidative damage. This study was conceived in order to analyze the RNA oxidative damage in the urine of patients undergoing operation for colorectal cancer (CRC), to compare with healthy controls, and correlate with the stage.
Materials And Methods: The study population was constituted by a group of 147 patients and a group of 128 healthy controls. Urine and blood samples were collected before the colonoscopy in all participants and 24 hours post-operatively for those who underwent surgery. Urine 8-hydroxyguanine (8-OHG) was determined as marker of RNA oxidation, and serum uric acid (UA) as antioxidant marker.
Results: Preoperatively, 8-OHG (ng/ml) values of CRC patients were found to be significantly higher than those of controls (p = 0.001). More specifically, stages II/III had significantly higher 8-OHG values (p < 0.001 and p = 0.007) than stages 0/I. Post-operatively, 8-OHG values were similar to controls (p = 0.053). Preoperatively, UA values (mg/dl) were significantly lower (p = 0.001), while postoperatively were similar to controls (p = 0.069).
Conclusion: Oxidative RNA damage occurs in CRC patients. Stages II/III are associated with higher values of 8-OHG than stages 0/I. 8-OHG could act as a marker for the identification of patients with advanced disease.
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| http://dx.doi.org/10.1080/08941939.2021.1904466 | DOI Listing |
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