AI Article Synopsis
- - The study investigates the effectiveness of hyaluronic acid (HA) coating on tracheal scaffolds to improve adhesion of mesenchymal stem cells (MSCs) for better tracheal repair in rabbits.
- - Researchers found that HA-coated scaffolds enhanced MSC adhesion and led to improved outcomes, such as wider airways and better mucosal regeneration compared to non-coated scaffolds.
- - The findings suggest that using HA coatings on tracheal scaffolds can significantly aid in regenerative medicine, particularly for treating tracheal defects.
Article Abstract
Background: Long segmental tracheal repair is challenging in regenerative medicine due to low adhesion of stem cells to tracheal scaffolds. Optimal transplantation of stem cells for tracheal defects has not been established. We evaluated the role of hyaluronic acid (HA) coating of tracheal scaffolds in mesenchymal stem cell (MSC) adhesion and tracheal regeneration in a rabbit model.
Methods: A three-dimensionally printed tubular tracheal prosthesis was incubated with dopa-HA-fluorescein isothiocyanate in phosphate-buffered saline for 2 days. MSCs were incubated with an HA-coated scaffold, and their adhesion was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. HA coated scaffolds with or without MSC seeding were transplanted at the circumferential tracheal defect in rabbits, and survival, rigid bronchoscopy, radiologic findings, and histologic findings were compared between the two groups.
Results: HA-coated scaffolds showed better MSC adhesion than non-coated scaffolds. The HA-coated scaffolds with MSC group showed a wider airway and greater mucosal regeneration compared to the HA-coated scaffolds without MSC group.
Conclusion: HA coating of scaffolds can promote MSC adhesion and tracheal regeneration.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012419 | PMC |
| http://dx.doi.org/10.1007/s13770-021-00335-2 | DOI Listing |
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