Validation of two UHPLC-MS/MS methods for fast and reliable determination of quinolone residues in honey.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess

Department of R&D, Altıparmak Food Industry and Trade Inc, Istanbul, Turkey.

Published: May 2021

Antibiotic usage for beekeeping, including quinolones, can lead to residues in honey and these residues usually result from the drugs used in the treatment of American or European foulbrood diseases. Residues in honey may cause allergic reactions as well as increased antibiotic resistance. Within this study, rapid and breakthrough analysis methods were developed using UHPLC-ESI-MS/MS and sample preparation processes were minimised. Practical, low cost, time-effective analysis was provided utilising 'dilute & shoot' methodology and a QuEChERS-based procedure has been developed alternatively for improved sensitivity. Recovery values were 85%-112% for the 'dilute & shoot' method, and 82%-117% for the modified QuEChERS method. For accuracy studies, RSD% values were between 0.7% and 13.4% for both methods in intra-day and inter-day precision studies. CCα (1.1 μg/kg and 0.6 μg/kg) and CCβ (1.2 μg/kg and 0.6 μg/kg) mean values were calculated for the 'dilute & shoot' and QuEChERS method, respectively. These novel methods achieved simultaneous quantification of 21 quinolones in 7 min with high specificity and were used to analyse 50 honey samples. Quinolone residues were found in samples, and enrofloxacin, danofloxacin, pipemidic acid, lomefloxacin, cinoxacin, and ciprofloxacin were quantified.

Download full-text PDF

Source
http://dx.doi.org/10.1080/19440049.2021.1881621DOI Listing

Publication Analysis

Top Keywords

residues honey
12
'dilute shoot'
12
quinolone residues
8
quechers method
8
residues
5
validation uhplc-ms/ms
4
methods
4
uhplc-ms/ms methods
4
methods fast
4
fast reliable
4

Similar Publications

Article Synopsis
  • Many pesticides used in agriculture can accumulate in the environment, exposing bees to multiple substances simultaneously, which is not commonly studied in research.
  • The study focused on the chronic effects of pesticide mixtures on honey bee worker's hemolymph, using concentrations found in their natural environment.
  • Results showed that acetamiprid decreased urea levels significantly, glyphosate had little effect, and tebuconazole, despite being considered safe, caused notable changes in several biochemical markers, indicating a need for further research on fungicides' impact on bees.
View Article and Find Full Text PDF

Nitroimidazoles in Albanian honey samples by LC-MS/MS analysis.

Food Addit Contam Part B Surveill

January 2025

Department of Chemical Sciences, Ariel University, Ariel, Israel.

This study employed QuEChERS extraction method combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to analyse honey samples from Albania for nitroimidazole residues. This approach allowed for the detection of trace-level residues, achieving sensitivity in the parts per billion range. Out of the 45 honey samples analysed, 6 exceeded the European Union's safety limit of 1 µg/kg, with metronidazole (MNZ) and dimetridazole (DMZ) being the predominant contaminants.

View Article and Find Full Text PDF

Background: The mite Varroa destructor is the most serious pest of the western honey bee (Apis mellifera) and a major factor in the global decline of colonies. Traditional control methods, such as chemical pesticides, although quick and temporarily effective, leave residues in hive products, harming bees and operators' health, while promoting pathogen resistance and spread. As a sustainable alternative, RNA interference (RNAi) technology has shown great potential for honey bee pest control in laboratory assays, but evidence of effectiveness in the field has been lacking.

View Article and Find Full Text PDF

Honeydew honey is less studied than nectar honey, although it is characterized by peculiar nutritional properties. This is mainly due to its challenging production, which leads to easy counterfeiting and difficult valorization. This contribution aims to provide a comprehensive characterization of the physico-chemical, palynological, functional, and food safety properties of a large sampling of honeydew honeys collected throughout Italy.

View Article and Find Full Text PDF

The bioaccumulation of pesticides in honeybee products (HBPs) should be studied for a number of reasons. The presence of pesticides in HBPs can provide new data on the risk related to the use of pesticides and their role in bee colony losses. Moreover, the degree of contamination of HBPs can lower their quality, weaken their beneficial properties, and, in consequence, may endanger human health.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!