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NEK1 deficiency affects mitochondrial functions and the transcriptome of key DNA repair pathways. | LitMetric

NEK1 deficiency affects mitochondrial functions and the transcriptome of key DNA repair pathways.

Mutagenesis

Departamento de Bioquímica e de Biologia Tecidual, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.

Published: July 2021

AI Article Synopsis

  • NEK1 plays a critical role in regulating DNA damage responses and mitochondrial functions, as shown by studies comparing NEK1 knockout (KO) and wild-type (WT) cells.
  • NEK1 KO cells exhibited increased cell death and sensitivity to certain genotoxic agents like methyl methanesulfonate, bleomycin, and UVC light, but showed resistance to cisplatin.
  • Changes in mitochondrial function were observed in NEK1 KO cells, including reduced mitophagy, elevated reactive oxygen species, and impaired mitochondrial repair processes, emphasizing NEK1's pivotal role in DNA repair and overall cell survival.

Article Abstract

Previous studies have indicated important roles for NIMA-related kinase 1 (NEK1) in modulating DNA damage checkpoints and DNA repair capacity. To broadly assess the contributions of NEK1 to genotoxic stress and mitochondrial functions, we characterised several relevant phenotypes of NEK1 CRISPR knockout (KO) and wild-type (WT) HAP1 cells. Our studies revealed that NEK1 KO cells resulted in increased apoptosis and hypersensitivity to the alkylator methyl methanesulfonate, the radiomimetic bleomycin and UVC light, yet increased resistance to the crosslinker cisplatin. Mitochondrial functionalities were also altered in NEK1 KO cells, with phenotypes of reduced mitophagy, increased total mitochondria, elevated levels of reactive oxygen species, impaired complex I activity and higher amounts of mitochondrial DNA damage. RNA-seq transcriptome analysis coupled with quantitative real-time PCR studies comparing NEK1 KO cells with NEK1 overexpressing cells revealed that the expression of genes involved in DNA repair pathways, such as base excision repair, nucleotide excision repair and double-strand break repair, are altered in a way that might influence genotoxin resistance. Together, our studies underline and further support that NEK1 serves as a hub signalling kinase in response to DNA damage, modulating DNA repair capacity, mitochondrial activity and cell fate determination.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262378PMC
http://dx.doi.org/10.1093/mutage/geab011DOI Listing

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