This study investigates the suitability of continuous hybrid fixed bed anaerobic filter reactor for treating sewage and agro-industrial digestate hydrothermal carbonization (HTC) products; hydrochar and HTC liquor (HTCL). The reactor was operated for 300 days under mesophilic conditions at different organic loading rates (OLR); maximum OLRs of 7.4 and 10 gCOD/L/d were reached while treating HTC liquor and slurry, respectively. 15 g/L hydrochar were added to the reactor as a supplement while treating HTCL solely thus increasing the biogas production up to 153%. The reactor was fed with HTCL and hydrochar with an increasing mixing ratio, and the co-digestion impact was dependent on hydrochar concentrations. The results of the study indicate that the hybrid fixed bed anaerobic filter reactor is a promising anaerobic digestion configuration for treating HTCL and overcoming the HTC upscaling challenges, and the suitability of digestate hydrochar utilization as supplement material for anaerobic digestion.
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http://dx.doi.org/10.1016/j.biortech.2021.124971 | DOI Listing |
bioRxiv
January 2025
Department of Molecular Physiology and Biophysics, School of Medicine, Vanderbilt University, Nashville, TN, 37232, USA.
Characterizing the movement of biomolecules in single cells quantitatively is essential to understanding fundamental biological mechanisms. RNA fluorescent in situ hybridization (RNA-FISH) is a technique for visualizing RNA in fixed cells using fluorescent probes. Automated processing of the resulting images is essential for large datasets.
View Article and Find Full Text PDFProc IEEE Int Symp Biomed Imaging
May 2024
Department of Electrical and Computer Engineering, Nashville, TN, USA.
Multiplex immunofluorescence (MxIF) imaging is a critical tool in biomedical research, offering detailed insights into cell composition and spatial context. As an example, DAPI staining identifies cell nuclei, while CD20 staining helps segment cell membranes in MxIF. However, a persistent challenge in MxIF is saturation artifacts, which hinder single-cell level analysis in areas with over-saturated pixels.
View Article and Find Full Text PDFZhonghua Bing Li Xue Za Zhi
February 2025
Department of Pathology, People's Hospital of Zhengzhou University/People's Hospital of Henan University, Zhengzhou 450043, China.
To investigate the expression pattern of pan-TRK protein in colorectal cancers with NTRK gene fusion and mismatch repair deficient (dMMR) and to analyze its molecular pathological characteristics. A total of 117 dMMR colorectal cancers diagnosed in the Department of Pathology of Henan Provincial People's Hospital, Zhengzhou, China from 2020 to 2023 were collected. Immunohistochemistry (IHC), fluorescence in situ hybridization (FISH) and DNA/RNA-based next-generation sequencing (NGS) were used to detect pan-TRK protein expression and fusion partner genes in tumors, and to further explore the correlation between pan-TRK staining patterns and partner genes.
View Article and Find Full Text PDFInt J Paediatr Dent
January 2025
Pediatric Dentistry Department, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.
Background: The growing demand for esthetic restorative materials highlights the need to evaluate their marginal accuracy and fracture resistance to ensure optimal clinical outcomes for primary molars.
Aim: The aim was to assess the vertical marginal gap distance and fracture resistance of esthetic restorative materials after cyclic loading.
Design: Forty extracted primary molars were randomly divided into four groups: Group I, stainless steel veneered crowns with tooth-colored material; Group II, prefabricated monolithic zirconia crowns; Group III, yttria-partially stabilized zirconia computer-aided design/computer-aided manufacturing (CAD/CAM) crowns; and Group IV, hybrid ceramic CAD/CAM crowns.
Entropy (Basel)
January 2025
Department of Mechanical and Aerospace Engineering, The University of Manchester, Manchester M1 3PL, UK.
This study investigates the flow field around a finite rectangular prism using both experimental and computational methods, with a particular focus on the influence of the turbulence approach adopted, the mesh resolution employed, and different subgrid length scales. Ten turbulence modelling and simulation approaches, including both 'scale-modelling' Reynolds-Averaged Navier-Stokes (RANS) models and 'scale-resolving' Delayed Detached Eddy Simulation (DDES), were tested across six different mesh resolutions. A case with sharp corners allows the location of the flow separation to be fixed, which facilitates a focus on the separated flow region and, in this instance, the three-dimensional interaction of three such regions.
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