Garlic is a well-known example of natural self-defence system consisting of an inactive substrate (alliin) and enzyme (alliinase) which, when combined, produce highly antimicrobial allicin. Increase of alliinase stability and its activity are of paramount importance in various applications relying on its use for in-situ synthesis of allicin or its analogues, e.g., pulmonary drug delivery, treatment of superficial injuries, or urease inhibitors in fertilizers. Here, we discuss the effect of temperature, pH, buffers, salts, and additives, i.e. antioxidants, chelating agents, reducing agents and cosolvents, on the stability and the activity of alliinase extracted from garlic. The effects of the storage temperature and relative humidity on the stability of lyophilized alliinase was demonstrated. A combination of the short half-life, high reactivity and non-specificity to particular proteins are reasons most bacteria cannot deal with allicin's mode of action and develop effective defence mechanism, which could be the key to sustainable drug design addressing serious problems with escalating emergence of multidrug-resistant (MDR) bacterial strains.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978267 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248878 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
NAT10 drives endometriosis progression through acetylation and stabilization of TGFB1 mRNA.
Mol Cell Endocrinol
December 2024
International Peace Maternity & Child Health Hospital, Shanghai Municipal Key Clinical Speciality, Institute of Embryo-Fetal Original Adult Disease, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China. Electronic address:
Endometriosis, a gynecological disorder marked by pelvic pain and infertility, has its pathogenesis and pathophysiology significantly influenced by epigenetics, as these factors have been well characterized. However, the role of RNA-mediated epigenetic regulation in endometriosis remains to be elucidated. In our study, we found that N4-acetylcytidine (acC) RNA modification and N-acetyltransferase 10 (NAT10) were significantly upregulated in endometrial lesions compared to eutopic endometrium.
View Article and Find Full Text PDFNSUN2-Mediated R-loop Stabilization as a Key Driver of Bladder Cancer Progression and Cisplatin Sensitivity.
Cancer Lett
December 2024
Department of Urology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310006, China; Cancer Center, Zhejiang University, Hangzhou, Zhejiang, 310058, China. Electronic address:
R-loops are critical structures that play pivotal roles in regulating genomic stability and modulating gene expression. This study investigates the interactions between the 5-methylcytosine (mC) methyltransferase NOP2/Sun RNA methyltransferase 2 (NSUN2) and R-loops in the transcriptional dynamics and damage repair process of bladder cancer (BCa) cells. We observed markedly elevated levels of R-loops in BCa cells relative to normal urothelial cells.
View Article and Find Full Text PDFInnovative spherical Fe-Mn layered double hydroxides (LDH) for the degradation of sulfisoxazole through activated periodate: Efficacy and mechanistic insights.
Environ Pollut
December 2024
Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China. Electronic address:
Advanced oxidation technology based on peroxides is widely regarded as an efficient method for treating emerging contaminants. However, the precise mechanism by which layered double hydroxides (LDHs) enhance oxidant activation requires further investigation. In this study, a spherical Fe-Mn LDH (S-FML) with improved crystallinity using a simple hydrothermal method.
View Article and Find Full Text PDFRemoval of phthalate esters by integrated adsorption and biodegradation using improved performance of lipase@MOFs.
Environ Pollut
December 2024
Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases and Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education Institutes, College of Life Sciences, Anhui Normal University, Wuhu 241000, Anhui, China. Electronic address:
Phthalate esters (PAEs) are broadly utilized as plasticizers in industrial products, posing a significant threat to ecological security and human health. Lipase is a kind of green biocatalyst with the ability to degrade PAEs, but its application is limited due to its low stability and poor reusability. Herein, lipase from Candida rugosa (CRL) was immobilized into an organic ligand replacement MOFs (MAF-507) and cysteine modification and glutaraldehyde cross-linking were simultaneously performed to synthesize immobilized lipase (Cys-CRL@GA@MAF-507) using a one-pot method.
View Article and Find Full Text PDFAcrylamide/Alyssum campestre seed gum hydrogels enhanced with titanium carbide: Rheological insights for cardiac tissue engineering.
Int J Biol Macromol
December 2024
Department of Petroleum Engineering, Chemistry and Chemical Engineering Research Center of Iran, Tehran, Iran; Polymer Synthesis Technology, School of Chemical Engineering, Aalto University, Espoo, Finland.
This study investigates the use of acrylamide and Alyssum campestre seed gum (ACSG) to create hydrogel composites with enhanced electrical and mechanical properties by incorporating titanium carbide (TiC). The composites were analyzed through techniques such as FTIR, SEM, TEM, TGA, swelling, rheology, tensile, electrical conductivity, antibacterial, and MTT assays. XRD analysis showed that 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!