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Article Synopsis
- Sodium influx due to hepatitis B virus (HBV) triggers the activation of the NLRP3 inflammasome in macrophages, working together with potassium efflux signals.
- The text describes a detailed protocol for performing patch-clamp assays to measure this sodium influx in THP-1 macrophages.
- It includes steps for differentiating, resting, and activating THP-1 macrophages, along with instructions for conducting whole-cell patch-clamp recordings.
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