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Association between 16S rRNA gene mutations and susceptibility to amikacin in Mycobacterium avium Complex and Mycobacterium abscessus clinical isolates. | LitMetric

AI Article Synopsis

  • - The study focused on the relationship between mutations in the 16S rRNA gene and resistance to amikacin in clinical isolates of nontuberculous mycobacteria (NTM) from patients with pulmonary disease.
  • - Out of 134 amikacin-resistant NTM isolates tested, a high percentage exhibited rrs mutations, especially those with higher drug resistance levels; specifically, 63% of isolates in Mycobacterium avium complex had mutations, while all resistant M. abscessus isolates displayed mutations.
  • - The researchers identified common mutations like A1408G, as well as new ones (C1496T and T1498A), and highlighted the significance of genetic and phenotypic

Article Abstract

We evaluated the association between 16S rRNA gene (rrs) mutations and susceptibility in clinical isolates of amikacin-resistant nontuberculous mycobacteria (NTM) in NTM-pulmonary disease (PD) patients. Susceptibility was retested for 134 amikacin-resistant isolates (minimum inhibitory concentration [MIC] ≥ 64 µg/ml) from 86 patients. Amikacin resistance was reconfirmed in 102 NTM isolates from 62 patients with either Mycobacterium avium complex-PD (MAC-PD) (n = 54) or M. abscessus-PD (n = 8). MICs and rrs mutations were evaluated for 318 single colonies from these isolates. For the 54 MAC-PD patients, rrs mutations were present in 34 isolates (63%), comprising all 31 isolates with amikacin MICs ≥ 128 µg/ml, but only three of 23 isolates with an MIC = 64 µg/ml. For the eight M. abscessus-PD patients, all amikacin-resistant (MIC ≥ 64 µg/ml) isolates had rrs mutations. In amikacin-resistant isolates, the A1408G mutation (n = 29) was most common. Two novel mutations, C1496T and T1498A, were also identified. The culture conversion rate did not differ by amikacin MIC. Overall, all high-level and 13% (3/23) of low-level amikacin-resistant MAC isolates had rrs mutations whereas mutations were present in all amikacin-resistant M. abscessus isolates. These findings are valuable for managing MAC- and M. abscessus-PD and suggest the importance of phenotypic and genotypic susceptibility testing.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969740PMC
http://dx.doi.org/10.1038/s41598-021-85721-5DOI Listing

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