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Develop a quantitative LC-MS/MS method for FDG, FDG-monophosphate, glucose and glucose-monophosphate in mouse tumor models to assist in validating the use of [F]FDG-positron emission tomography (PET) imaging for anticancer therapies in a clinical setting. Analytes were isolated from tumors by protein precipitation and detected on a Sciex API-5500 mass spectrometer. Improved assay robustness and selectivity were achieved through chromatographic separation of FDG-monophosphate from glucose-monophosphate, selection of a unique ion transition and incorporation of stable isotope labeled internal standards. In a mouse JIMT-1 tumor model, FDG-monophosphate levels measured by LC-MS/MS correlated with [F]FDG-PET imaging results. LC-MS/MS analysis of FDG-monophosphate accumulation in tumors is a cost-effective tool to gauge the translational potential of [F]FDG-PET imaging as a noninvasive biomarker in clinical studies.
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| http://dx.doi.org/10.4155/bio-2020-0326 | DOI Listing |
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