Chilling stress (CS) is an important limiting factor for the growth and development of passion fruit () in winter in South China. However, little is known about how the passion fruit responds and adapts to CS. In this study, we performed transcriptome sequencing of cold-susceptible cultivar Huangjinguo (HJG) and cold-tolerant cultivar Tainong 1 (TN1) under normal temperature (NT) and CS conditions, and a total of 47,353 unigenes were obtained by seven databases. Using differentially expressed unigenes (DEGs) analysis, 3,248 and 4,340 DEGs were identified at two stages, respectively. The Gene Ontology (GO) enrichment analysis showed that the DEGs were mainly related to phosphorylation, membrane protein, and catalytic activity. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, the unigenes of plant-pathogen interaction, plant hormone signal transduction and fatty acid metabolism were enriched. Then, the 12,471 filtered unigenes were clustered into eight co-expression modules, and two modules were correlated with CS. In this two modules, 32 hub unigenes were obtained. Furthermore, the unigenes related to CS were validated using quantitative real-time PCR (RT-qPCR). This work showed that the expression levels of CS-related unigenes were very different in two passion fruit cultivars. The results provide information for the development of passion fruit with increased chilling tolerance.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936571 | PMC |
| http://dx.doi.org/10.7717/peerj.10977 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Rapid PCR-LAMP Assay for the Early Detection of Lasiodiplodia theobromae from Basal Stem Rot-Infected Passion Fruit Plants.
Mol Biotechnol
January 2025
Guangxi Subtropical Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530001, Guangxi, China.
Lasiodiplodia theobromae is an emerging threat and the main pathogenic fungi associated with basal stem rot of passion fruit in Guangxi Zhuang Autonomous Region, China. Current pathogen identification protocols are labor-intensive and time-consuming, emphasizing the need for more efficient methods to enable precise surveillance of L. theobromae for early detection and warning.
View Article and Find Full Text PDFMelatonin alleviated chilling injury of cold-stored passion fruit by modulating cell membrane structure via acting on antioxidant ability and membrane lipid metabolism.
Curr Res Food Sci
December 2024
College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China.
Fresh passion fruit is sensitive to chilling injury (CI) during storage at improper low temperature of 5 °C, which lowers the fruit quality and limits its shelf life. The present study aimed to determine the impacts of melatonin on CI development of passion fruit in relation to antioxidant ability and membrane lipid metabolism during refrigeration. In present study, passion fruit was treated with 0.
View Article and Find Full Text PDFPassion fruit seed extract protects hydrogen peroxide-induced cell damage in human retinal pigment epithelium ARPE-19 cells.
Sci Rep
January 2025
Department of Biochemistry and Molecular Biology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Age-related macular degeneration (AMD) is a major cause of vision loss among adults. We investigated the protective effects of passion fruit seed extract (PFSE) and its rich polyphenol piceatannol in an AMD cell model in which human retinal pigment epithelial ARPE-19 cells were exposed to hydrogen peroxide (HO). Using a cell viability WST-8 assay, we revealed that PFSE and piceatannol increased the cellular viability of ARPE-19 cells by 130% and 133%, respectively.
View Article and Find Full Text PDFIntegrated metabolomics and proteomics analysis of anthocyanin biosynthesis regulations in passion fruit (Passiflora edulis) pericarp.
Plant Physiol Biochem
December 2024
Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, China.
Anthocyanin is the primary color-developing component in the pericarp of the passion fruit. Although the pericarp of the passion fruit is anticipated to be a significant source of anthocyanin, however, information regarding anthocyanin biosynthesis in the passion fruit pericarp remains unexplored. Based on metabolomics analysis, a total of five anthocyanins were identified in the purple-skinned passion fruit pericarp, among which three anthocyanins, petunidin-3-O-arabinoside, geranylgeranyl-3,5-O-diglucoside, and petunidin-3-O-rutinoside, play key roles in the coloration of the passion fruit pericarp.
View Article and Find Full Text PDFIdentification and Molecular Characterization of Telosma Mosaic Virus (TelMV) and East Asian Passiflora Virus (EAPV) from Patchouli in China.
Viruses
November 2024
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
Patchouli is a valuable medicinal herb and cash crop in China, but viral infections cause significant yield losses. This study identified six viruses in patchouli transcriptome data, including the first-ever detection of East Asian Passiflora Virus (EAPV) in patchouli. RT-PCR validated three viruses from diseased patchouli plants in Haikou, China: telosma tosaic virus (TelMV), broad bean wilt virus-2 (BBWV-2), and pogostemom alphacytorhabdovirus 1 (PogACRV1_Pog).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!