AI Article Synopsis
- Rolling circle replication (RCR) is an efficient enzymatic process used for amplifying biomolecules, making it a valuable tool for biosensing.
- Recent advancements have expanded RCR's applications to bioimaging and drug delivery, allowing for improved targeting of biological pathways.
- The review highlights the fundamentals of RCR, its latest detection methods and delivery systems, and discusses existing challenges as well as future opportunities in RCR-based biotechnology.
Article Abstract
Rolling circle replication (RCR), including rolling circle amplification (RCA) and rolling circle transcription (RCT), is an isothermal enzymatic reaction. Because of its high amplification efficiency, RCR is a powerful biosensing tool for detecting biomolecules. In recent years, RCR has also been extended to the field of bioimaging to better understand biological pathways. Furthermore, RCR provides a simple technique to design and generate DNA/RNA structures with unique advantages in delivering drugs and enhanced targeting ability. In this review, we introduce the fundamentals of RCR and describe the most recent advances in RCR-based detection methods and delivery vehicles for biosensing, bioimaging, and biomedicine. Finally, some challenges and further opportunities of RCR-based biotechnology are discussed.
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| http://dx.doi.org/10.1016/j.tibtech.2021.02.007 | DOI Listing |
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