AI Article Synopsis
- This study examined how paraproteins, specifically IgA, can lead to false positive results in Kinetic Interaction of Microparticles in Solution (KIMS) immunoassays commonly used in clinical laboratories.
- Researchers analyzed various patient samples and controls using multiple laboratory techniques to identify the cause of the interference.
- The findings point to IgA paraprotein being responsible for incorrect measurements of three therapeutic drugs, highlighting the significance of understanding such interferences in clinical testing.
Article Abstract
Immunoassays are commonly used by the clinical laboratory, but paraproteins can occasionally produce erroneous results. In this study, we investigated the cause of apparent false positive results for multiple Kinetic Interaction of Microparticles in Solution (KIMS) immunoassays. Patient controls and samples containing the interference were analyzed using automated chemistry platforms, gel electrophoresis, immunofixation, affinity chromatography, and size exclusion chromatography. Our results show that IgA paraprotein caused false positive results for the KIMS measurement of three therapeutic drugs. To our knowledge, this is the first report of IgA paraprotein-causing immunoassay interference. The clinical implications of this interference are discussed.
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| http://dx.doi.org/10.1016/j.clinbiochem.2021.03.001 | DOI Listing |
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