AI Article Synopsis
- Iota toxin is produced by Clostridium perfringens, featuring an enzymatic component (Ia) that inhibits actin polymerization and a membrane-binding component (Ib) that creates a pore for Ia translocation.
- The structure of the Ia-Ib-pore complex has been resolved using cryo-electron microscopy, but questions about Ia's translocation mechanisms remain.
- A new method developed for preparing Ia-bound Ib-pore samples improves structural analysis at high resolution and reveals two different states of the Ib-pore, which can enhance studies of iota toxin and similar toxins.
Article Abstract
Iota toxin, a type of A-B toxin produced by Clostridium perfringens, comprises an enzymatic component (Ia) and a membrane-binding component (Ib). The translocation of Ia to the target cell via the pore formed by Ib allows it to function as an ADP-ribosyltransferase that inhibits actin polymerization in the host cell. The structure of Ia-bound Ib-pore has been determined using cryo-electron microscopy (cryo-EM), thereby elucidating the mechanism of the initial Ia translocation; however, open questions regarding Ia translocation still exist. In this chapter, we describe a new method of preparing Ia-bound Ib-pore complex samples for structural analysis at high resolution using cryo-EM. This method is different from previously reported methods for other A-B toxins. Consequently, it produces Ib-pore with two different states with short and long membrane-spanning β-barrel stem. We expect that this method will be useful in functional and structural studies of iota toxin and other binary toxins.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/bs.mie.2021.01.032 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Influence of sewage effluent discharge on putative pathogen community in drinking water sources: insights from full-length 16S rRNA gene amplicon sequencing.
J Water Health
January 2025
Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto University Katsura, Nishikyo, Kyoto 615-8540, Japan.
The discharge of sewage effluent is a major source of microbial contamination in drinking water sources, necessitating a comprehensive investigation of its impact on pathogenic bacterial communities. This study utilized full-length 16S rRNA gene amplicon sequencing to identify putative pathogenic bacteria and analyze their community structures in drinking water sources subjected to different levels of fecal pollution: urban rivers with low, moderate, and high sewage effluent mixing ratios, and mountain streams with minimal human impact. The sewage effluent itself was also analyzed.
View Article and Find Full Text PDFA comparative evaluation of antibiotic and synbiotic supplementation on production performance and necrotic enteritis severity in broilers during an experimental necrotic enteritis challenge.
Front Physiol
January 2025
Department of Poultry Science, University of Georgia, Athens, GA, United States.
The ban on antibiotics in the poultry diet resulted in re-emergence of several infectious diseases including necrotic enteritis (NE). These infectious diseases are leading to poor health and welfare as well as production and economic loss. Synbiotic could be a potential candidate to replace the antibiotics in poultry diet.
View Article and Find Full Text PDFenterotoxin-claudin pore complex: Models for structure, mechanism of pore assembly and cation permeability.
Comput Struct Biotechnol J
December 2024
Clinical Physiology/Nutritional Medicine, Department of Gastroenterology, Rheumatology and Infectious Diseases, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany.
The pore-forming enterotoxin (CPE), a common cause of foodborne diseases, facilitates Ca influx in enterocytes, leading to cell damage. Upon binding to certain claudins (e.g.
View Article and Find Full Text PDFMolecular Epidemiology of Type F Among Diarrheal Patients and Virulence-Resistance Dynamics - 11 Provinces, China, 2024.
China CDC Wkly
January 2025
Department of Clinical Laboratory, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, China.
Introduction: Type F () represents a significant pathogen in human gastrointestinal diseases, primarily through its gene encoding enterotoxin (CPE). This investigation examined the prevalence, antimicrobial resistance patterns, and genetic characteristics of Type F within the Chinese population.
Methods: The study analyzed 2,068 stool samples collected from 11 provincial hospitals in 2024.
Point-of-care ultrasound in the diagnosis of hepatic gas gangrene.
J Ultrasound
January 2025
Argentinian Critical Care Ultrasonography Association (ASARUC), Buenos Aires, Argentina.
Hepatic gas gangrene (HGG) is a rare but life-threatening condition typically caused by anaerobic bacteria such as Clostridium perfringens, though Gram-negative bacteria like Escherichia coli and Klebsiella species have also been implicated. Traditionally diagnosed via computed tomography (CT), point-of-care ultrasound (POCUS) has emerged as a valuable tool in critical care settings for its non-invasive, bedside utility. We report the case of a 51-year-old female with choledochal syndrome secondary to cholangiocarcinoma who developed HGG following left extended hepatectomy and biliary reconstruction.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!