sp. nov., isolated from humic soil on bird's nest fern ( L.).

Int J Syst Evol Microbiol

Microbial Diversity and Utilization Research Team, Thailand Bioresource Research Center, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand.

Published: June 2019

A novel member of the actinobacteria, designated strain A-T 0013, was isolated from humic soil on a bird's nest fern ( L.) collected from Khao Yai National Park in Thailand. According the results of a polyphasic taxonomic study, A-T 0013 had characteristics typical of members of the genus . The 16S rRNA gene sequence indicated that A-T 0013 shared ≤98 % sequence similarity with all members of the genus . The most closely related species was IFM 10200 (97.92 % sequence similarity). The average nucleotide identity based on blast (ANIb) value with IFM 10200 was 76.81 %. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained ribose, arabinose and galactose. The predominant menaquinone was MK-9(H). The predominant fatty acids were C, Cω9, summed feature 3 (Cω7/Cω6), and C 10-methyl. Mycolic acid was present. The polar lipid profile for this strain ncluded diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The G+C content of the genomic DNA was 66.1 mol%. Differentiation of A-T 0013 from the most closely related species, IFM 10200, was evident from digital DNA-DNA hybridization values of 21.8 %. On the basis of the results of comparative analysis of phenotypic, chemotaxonomic and genotypic data, strain A-T 0013 is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A-T 0013 (=TBRC 11910=NBRC 114549).

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http://dx.doi.org/10.1099/ijsem.0.004746DOI Listing

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