Purpose: Hydatid disease is one of the neglected and challenging (for diagnosis as well as for treatment) parasitic diseases. Along with adverse effect on animal's health leading to production losses, hydatidosis is also associated with huge economic losses. The present study was envisaged with an aim to assess the phylogeny and pathological changes due to natural hydatid cysts in lungs and liver of slaughtered buffaloes in north India.
Methods: A total of 137 slaughtered buffaloes intended for human consumption were screened for the presence of cysts. The retrieved cysts were confirmed molecularly based on the amplification of mitochondrial cytochrome oxidase 1 gene (mtCO1), exhibiting a product size of approximately 446 bp. The samples collected from infected lungs and liver were subjected to histopathological examination.
Results: The hydatid cysts were recorded in 25 (18.2%) animals. Phylogenetic analysis revealed the isolated strain to be closely related to Echinococcus granulosus sensu stricto (G1) genotype. The nucleotide diversity (π) obtained was 0.014685, whereas, Tajima's D was negative (- 2.796053), which indicated purifying selection or recent population expansion. Histopathologically, in the infected lungs, fibrosis and inflammatory reaction comprising of mononuclear cells and fibroblasts around the thick coat of granulation tissue were observed. Marked calcified masses and necrosis were also observed in the calcified cysts. However, in case of infected livers, Kupffer cell hyperplasia, degeneration of hepatocytes, fibrosis and inflammatory cells were most commonly observed around the hydatid cysts.
Conclusion: The findings of the present study are of significant veterinary and medical importance owing to economic and public health impact of G1 genotype of E. granulosus.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11686-021-00348-w | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prevalence and histopathological analysis of cystic echinococcosis in ruminants of District Narowal, Pakistan: focus on pulmonary involvement.
Rev Bras Parasitol Vet
January 2025
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
A total of 384 animals (sheep, goat, cattle, and buffalo) were examined for the presence of hydatid cysts only in the lungs. The lung tissue samples associated with the hydatid cyst were collected immediately after slaughter, followed by fixation in 10% formalin. The fixed tissue was subjected to paraffin embedding technique.
View Article and Find Full Text PDFThe present study aimed to evaluate the histologic, histochemical, and immunohistochemical changes in buffalo livers with cystic echinococcosis. Noninfected and infected livers were collected from the freshly slaughtered buffalo at the Aligarh abattoir. Small pieces of both infected and noninfected livers ( = 5) were cut and processed for histologic and histochemical studies.
View Article and Find Full Text PDFInfection survey, molecular, pathogenicity, and morphological characteristics of Sarcocystis species naturally infected water buffaloes (Bubalus bubalis) in Egypt.
BMC Vet Res
December 2024
Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girl's Branch), Cairo, Egypt.
Background: Sarcocystosis is a parasitic disease found worldwide, resulting from various Sarcocystis species. The current research was carried out in three significant economic areas in Egypt: Greater Cairo, the Nile Delta, and Upper Egypt. It aimed to investigate the occurrence of Sarcocystis spp.
View Article and Find Full Text PDFDiagnosis and hormonal treatment of cystic ovaries associated with uterine disorders in Egyptian buffaloes: ultrasonography, histopathological and serological investigations.
Trop Anim Health Prod
November 2024
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Sohag University, P.O. Box 82524, Sohag, Egypt.
Article Synopsis
- Cystic ovarian disease (COD) is a reproductive issue that affects Egyptian buffaloes postpartum, leading to significant economic challenges.
- The study focused on the effectiveness of the GnRH/PGF2α treatment protocol for improving fertility and reducing economic losses linked to COD.
- Results showed that treated buffaloes had improved hormonal profiles, decreased inflammatory markers, and notable structural changes in their reproductive tissues compared to untreated buffaloes.
Unveiling the hidden threat: investigating gastrointestinal parasites and their costly impact on slaughtered livestock.
Rev Bras Parasitol Vet
October 2024
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Article Synopsis
- A study in Narowal, Punjab, found that 72.92% of ruminants slaughtered were infected with gastrointestinal parasites, with goats having the highest infection rate at 78.63%.
- Female ruminants experienced significantly higher infection rates (85.62%) than males (65.13%).
- The research revealed a severe economic impact, estimating annual losses of approximately Pak. Rs. 405.09 million (around USD 1.43 million) due to organ condemnation from GI parasites.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!