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Molecular Characterization of Hydatid Cysts Cases in a Wild Boar and Mule in Turkey. | LitMetric

Molecular Characterization of Hydatid Cysts Cases in a Wild Boar and Mule in Turkey.

Turkiye Parazitol Derg

Fırat Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Klinik Öncesi Bilimler Bölümü, Elazığ, Türkiye

Published: March 2021

AI Article Synopsis

Article Abstract

Objective: Cystic echinococcosis (CE) is a zoonotic infection that affects humans, livestock and wild animals through the larval form of (s.l.). Molecular and taxonomic studies carried out in the recent years accept that Echinococcus granulosus s.l., a complex of 5 cryptic species, causes CE. In this study, we performed morphological and molecular characterisation of cyst isolates obtained from a wild boar and mule naturally infected with hydatid cyst.

Methods: After gDNA isolation, the mitochondrial cytochrome oxidase subunit 1 (mt-) gene region was amplified by polymerase chain reaction (PCR) with specific primers. The amplified mt- PCR products were purified and one-way DNA sequence analysis was performed.

Results: Comparison of the partial sequences of mt gene from the hydatid cyst isolates with that of reference sequences in GenBank revealed 100% similarity with (G1-G3) sequences.

Conclusion: To the best of our knowledge, this is the first study to determine the molecular characterisation of Echinococcus species in a wild boar in Turkey.

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Source
http://dx.doi.org/10.4274/tpd.galenos.2020.6818DOI Listing

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