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Direct Tissue PCR and Genotyping for Species Identification in a Case of Laryngeal Blastomycosis. | LitMetric

Direct Tissue PCR and Genotyping for Species Identification in a Case of Laryngeal Blastomycosis.

Ear Nose Throat J

Integrated Research and Development Laboratory, Marshfield Clinic Research Institute, Marshfield Clinic Health System, Marshfield, WI, USA.

Published: April 2023

AI Article Synopsis

  • Otolaryngologic infections caused by certain species are rare and mostly found in specific geographic areas.
  • This case study discusses a laryngeal blastomycosis that initially presented as a slow worsening of voice quality (dysphonia) and was diagnosed using histopathology and molecular techniques instead of traditional fungal culture.
  • After one month of antifungal treatment, all symptoms improved, highlighting the importance of rapid molecular methods for understanding the disease's clinical variations.

Article Abstract

Otolaryngologic manifestations of infection with species are extremely rare and restricted geographically to recognized endemic regions. Here, we describe a case of laryngeal blastomycosis that presented as slowly progressive dysphonia. While a preliminary diagnosis was made using routine histopathology, a species identification of was made using polymerase chain reaction amplification and rapid genotyping without the need for fungal culture. All symptoms resolved following 1 month of antifungal therapy. Rapid molecular differentiation of from provides important insights into pathogenesis given recent recognition of differences in clinical spectra.

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Source
http://dx.doi.org/10.1177/0145561321991342DOI Listing

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