AI Article Synopsis
- Aldehydes are found in tobacco smoke and urban air pollution, and they can also form in the body during lipid peroxidation.
- These aldehydes can interact with DNA, creating harmful exocyclic adducts that serve as biomarkers for various health issues, including cancer and neurodegenerative diseases.
- A new, validated micro-HPLC-Electro Spray Ionization-MS/MS method is introduced for accurately measuring a specific DNA adduct, 1,N-propanodGuo, which results from acetaldehyde or crotonaldehyde reacting with DNA.
Article Abstract
Aldehydes are abundantly present in tobacco smoke and in urban air pollution and are endogenously generated as products of the lipid peroxidation process. These molecules can react with DNA bases forming mutagenic exocyclic adducts, which have been used as biomarkers of aldehyde exposure and as potential tools for the study of inflammation, metal storage diseases, neurodegenerative disorders, and cancer. High-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) provides a highly precise, specific and ultrasensitive method for the detection of exocyclic DNA adducts. Here we present and describe a validated micro-HPLC-Electro Spray Ionization (ESI)-MS/MS method for the quantification of 1,N-propanodGuo, an adduct produced following the reaction between 2'-deoxyguanosine and acetaldehyde or crotonaldehyde.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-1278-1_18 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!